10XP

Streptomyces lividans DnaA DI protein in the I222 space group.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-08-02
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	I 2 2 2
Unit cell lengths	37.739, 56.347, 153.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.440 - 2.000
R-factor	0.216
Rwork	0.211
R-free	0.26110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.440	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.066	0.372
Rpim	0.015	0.084
Number of reflections	11469	827
<I/σ(I)>	26.4	6.8
Completeness [%]	99.8	100
Redundancy	18.5	19.1
CC(1/2)	1.000	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2% (w/v) PEG 400; 100 mM Tris base/hydrochloric acid, pH 8.5; 2M lithium sulfate