10XO

Streptomyces lividans DnaA DI protein in the P6(1)22 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-18
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 61 2 2
Unit cell lengths	57.460, 57.460, 99.032
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.230 - 2.000
R-factor	0.232
Rwork	0.228
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.678
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.230	2.050
High resolution limit [Å]	2.000	2.000
Rmeas	0.072	1.105
Rpim	0.006	0.091
Number of reflections	7049	510
<I/σ(I)>	85	8.1
Completeness [%]	99.9	100
Redundancy	161.5	146.7
CC(1/2)	0.999	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.2 M sodium chloride; 0.1 M sodium acetate trihydrate, pH 4.6