10XL

Pseudonocardia thermophila DnaA DI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-13
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 1 21 1
Unit cell lengths	35.597, 51.823, 35.933
Unit cell angles	90.00, 91.73, 90.00

Refinement procedure

Resolution	22.450 - 1.650
R-factor	0.192
Rwork	0.190
R-free	0.21260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.221
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.450	1.680
High resolution limit [Å]	1.650	1.650
Rmeas	0.052	0.586
Rpim	0.011	0.163
Number of reflections	15815	770
<I/σ(I)>	36.3	3.6
Completeness [%]	100.0	100
Redundancy	21.5	12.5
CC(1/2)	1.000	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	1 M sodium citrate tribasic; 100 mM imidazole/hydrochloric acid, pH 8.0