10XK

Bifidobacterium primatium DnaA DI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-12-04
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 1 21 1
Unit cell lengths	55.927, 124.131, 63.747
Unit cell angles	90.00, 97.70, 90.00

Refinement procedure

Resolution	22.450 - 2.400
R-factor	0.1959
Rwork	0.193
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.863
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.450	2.490
High resolution limit [Å]	2.400	2.400
Rmeas	0.064	0.405
Rpim	0.017	0.119
Number of reflections	33680	3544
<I/σ(I)>	25.4	5.2
Completeness [%]	99.8
Redundancy	12
CC(1/2)	0.999	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	9% (w/v) PEG 8000; 50 mM Sodium cacodylate/HCl, pH 6.5; 100 mM Calcium Acetate; 12.5% glycerol