10XH
Bifidobacterium bifidum DnaA DI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-19 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.931, 50.965, 47.724 |
| Unit cell angles | 90.00, 120.12, 90.00 |
Refinement procedure
| Resolution | 23.670 - 1.900 |
| R-factor | 0.1862 |
| Rwork | 0.182 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.879 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.670 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.043 | 0.482 |
| Rpim | 0.011 | 0.122 |
| Number of reflections | 12871 | 895 |
| <I/σ(I)> | 40.7 | 6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.2 | 15.3 |
| CC(1/2) | 1.000 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% (v/v) 1,4-butanediol; 100 mM sodium acetate/acetic acid, pH 4.5 |
