10XG

Corynebacterium bovis DnaA DI S89C disulfide-linked dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-08-29
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 61 2 2
Unit cell lengths	58.686, 58.686, 93.251
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.310 - 1.600
R-factor	0.1971
Rwork	0.193
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.587
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.310	1.630
High resolution limit [Å]	1.600	1.600
Rmeas	0.097	0.781
Rpim	0.019	0.208
Number of reflections	13172	629
<I/σ(I)>	18.7	2.3
Completeness [%]	100.0	100
Redundancy	22.8	12.8
CC(1/2)	0.999	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	21% (w/v) PEG 3000; 70 mM Tris base/Hydrochloric acid, pH 7.0; 140 mM sodium chloride