10XF
Corynebacterium bovis DnaA DI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-07-24 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 58.577, 58.577, 93.706 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.370 - 1.800 |
| R-factor | 0.1919 |
| Rwork | 0.188 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.724 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.310 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.106 | 1.257 |
| Rpim | 0.022 | 0.258 |
| Number of reflections | 9348 | 546 |
| <I/σ(I)> | 23.2 | 2.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 22.6 | 22.3 |
| CC(1/2) | 0.999 | 0.929 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.0 M Ammonium Citrate dibasic; 0.1 M Sodium Acetate trihydrate, pH 4.6 |
