10XE

Corynebacterium diphtheriae DnaA DI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88560
Spacegroup name	P 21 21 21
Unit cell lengths	49.682, 54.982, 55.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.120 - 0.960
R-factor	0.1418
Rwork	0.141
R-free	0.16200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.120	0.980
High resolution limit [Å]	0.960	0.960
Rmeas	0.063	1.599
Rpim	0.012	0.345
Number of reflections	87895	3100
<I/σ(I)>	26.4	1.9
Completeness [%]	93.9	68.1
Redundancy	25.5	19.9
CC(1/2)	1.000	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	800 mM potassium phosphate dibasic; 100 mM HEPES/sodium hydroxide, pH 7.5; 800 mM sodium phosphate dibasic