10WZ

Rhodococcus hoagii DnaA DI in the P6(2)22 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU PhotonJet-S
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-29
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	P 62 2 2
Unit cell lengths	61.100, 61.100, 132.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.420 - 3.000
R-factor	0.2201
Rwork	0.218
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.693
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.690	3.180
High resolution limit [Å]	3.000	3.000
Rmeas	0.132	0.483
Rpim	0.033	0.114
Number of reflections	3312	517
<I/σ(I)>	16.2	4
Completeness [%]	99.7	100
Redundancy	15.9	17.4
CC(1/2)	0.999	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% (w/v) PEG 1000, 100 mM Sodium acetate/acetic acid pH 4.5, 200 mM Zinc acetate