10VC

Crystal structure of GeoCas9 HNH domain bound to anti-CRISPR AcrIIC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-17
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	83.529, 89.252, 86.940
Unit cell angles	90.00, 93.65, 90.00

Refinement procedure

Resolution	29.590 - 1.790
R-factor	0.1963
Rwork	0.194
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (2.0_5885)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.590	1.810
High resolution limit [Å]	1.790	1.790
Number of reflections	119866	234
<I/σ(I)>	2.5
Completeness [%]	100.0
Redundancy	5.7
CC(1/2)	0.997	0.341

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M Sodium malonate pH 6.0, 20% w/v PEG 3,350