10TH
Crystal structure of the PXL domain of the human promyelocytic leukemia (PML) protein isoform 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-07-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979338 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.343, 114.343, 102.678 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 21.610 - 3.010 |
| R-factor | 0.171 |
| Rwork | 0.167 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.008 | 3.390 |
| High resolution limit [Å] | 3.007 | 3.007 |
| Rmerge | 0.460 | 3.753 |
| Rmeas | 0.480 | 3.918 |
| Rpim | 0.135 | 1.113 |
| Number of reflections | 9767 | 5953 |
| <I/σ(I)> | 6 | 1.4 |
| Completeness [%] | 93.3 | 67.3 |
| Redundancy | 12.5 | 12.2 |
| CC(1/2) | 0.992 | 0.324 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 2 M NaCl, 100 mM TrisHCl, and 200 mM MgCl2 |
