10OQ

FGFR2 mutant D650V with compound 6

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 31 2 1
Unit cell lengths	96.743, 96.743, 145.622
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.966 - 1.980
R-factor	0.20617
Rwork	0.204
R-free	0.25144
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.700
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.966	2.010
High resolution limit [Å]	1.980	1.980
Rmerge	0.188
Number of reflections	55606	2700
<I/σ(I)>	9.6
Completeness [%]	100.0	99.6
Redundancy	20.6	19.5
CC(1/2)	0.999	0.329

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	293	22% PEG 4,000, 200 mM Ammonium Sulfate, 100 mM Sodium Citrate pH 5.6