10LI
D-Ornithine/D-lysine decarboxylase complexed with putrescine and agmatine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-30 |
| Detector | DECTRIS PILATUS 1M |
| Wavelength(s) | 1.54187 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.413, 50.556, 73.281 |
| Unit cell angles | 90.00, 120.69, 90.00 |
Refinement procedure
| Resolution | 36.630 - 1.670 |
| R-factor | 0.1434 |
| Rwork | 0.141 |
| R-free | 0.18020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.842 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.610 | 1.600 |
| High resolution limit [Å] | 1.572 | 1.572 |
| Number of reflections | 48055 | 167 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 78.4 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.965 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, pH 7.5, 0.2 M NaOAc, 22% PEG 4000 |
