100D
CRYSTAL STRUCTURE OF THE HIGHLY DISTORTED CHIMERIC DECAMER R(C)D(CGGCGCCG)R(G)-SPERMINE COMPLEX-SPERMINE BINDING TO PHOSPHATE ONLY AND MINOR GROOVE TERTIARY BASE-PAIRING
Experimental procedure
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 263 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS-NICOLET |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 23.980, 40.770, 44.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.145 |
Rwork | 0.145 |
RMSD bond length | 0.015 |
RMSD bond angle | 3.800 |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.900 |
Rmerge | 0.058 * |
Number of reflections | 2365 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | SPERMINE_HCL | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | decamer duplex | 1 (mM) | |
2 | 1 | drop | sodium cacodylate | 40 (mM) | |
3 | 1 | drop | spermine tetrachloride | 2.0 (mM) | |
4 | 1 | reservoir | MPD | 45 (%) |