+Open data
-Basic information
Entry | Database: PDB / ID: 5b4y | ||||||
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Title | Crystal structure of the LA12 fragment of ApoER2 | ||||||
Components | Low-density lipoprotein receptor-related protein 8 | ||||||
Keywords | SIGNALING PROTEIN / signal transduction | ||||||
Function / homology | Function and homology information ammon gyrus development / reelin receptor activity / positive regulation of CREB transcription factor activity / very-low-density lipoprotein particle receptor activity / ventral spinal cord development / reelin-mediated signaling pathway / high-density lipoprotein particle binding / low-density lipoprotein particle receptor activity / layer formation in cerebral cortex / positive regulation of dendritic spine morphogenesis ...ammon gyrus development / reelin receptor activity / positive regulation of CREB transcription factor activity / very-low-density lipoprotein particle receptor activity / ventral spinal cord development / reelin-mediated signaling pathway / high-density lipoprotein particle binding / low-density lipoprotein particle receptor activity / layer formation in cerebral cortex / positive regulation of dendritic spine morphogenesis / cellular response to cholesterol / positive regulation of dendrite development / dendrite morphogenesis / cargo receptor activity / microtubule associated complex / retinoid metabolic process / regulation of innate immune response / Platelet sensitization by LDL / apolipoprotein binding / kinesin binding / positive regulation of protein tyrosine kinase activity / Retinoid metabolism and transport / caveola / modulation of chemical synaptic transmission / lipid metabolic process / cellular response to growth factor stimulus / endocytosis / cytokine-mediated signaling pathway / calcium-dependent protein binding / positive regulation of peptidyl-tyrosine phosphorylation / transmembrane signaling receptor activity / amyloid-beta binding / chemical synaptic transmission / regulation of apoptotic process / receptor complex / response to xenobiotic stimulus / axon / dendrite / neuronal cell body / synapse / calcium ion binding / cell surface / signal transduction / proteolysis / extracellular space / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Nogi, T. / Tabata, S. / Hirai, H. / Yasui, N. / Takagi, J. | ||||||
Citation | Journal: To Be Published Title: Crystal structure of the ectodomain from a LDLR close homologue in complex with its physiological ligand. Authors: Hirai, H. / Yasui, N. / Yamashita, K. / Tabata, S. / Yamamoto, M. / Takagi, J. / Nogi, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5b4y.cif.gz | 31.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5b4y.ent.gz | 19.2 KB | Display | PDB format |
PDBx/mmJSON format | 5b4y.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b4/5b4y ftp://data.pdbj.org/pub/pdb/validation_reports/b4/5b4y | HTTPS FTP |
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-Related structure data
Related structure data | 5b4xC 3a7qS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 9624.260 Da / Num. of mol.: 1 / Fragment: UNP RESIDUES 42-124 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: LRP8, APOER2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q14114 | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Sequence details | UNP Q14114 shows Natural variant at this position | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.86 Å3/Da / Density % sol: 33.95 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 20%(wt./vol.) PEG monomethylether 2000, 100 mM Tris-Cl (pH 7.0) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-17A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 4, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→41.8 Å / Num. obs: 6043 / % possible obs: 99.9 % / Redundancy: 5.2 % / CC1/2: 0.995 / Rmerge(I) obs: 0.086 / Net I/σ(I): 9.5 |
Reflection shell | Resolution: 1.9→1.94 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3A7Q Resolution: 1.9→33.28 Å / Cor.coef. Fo:Fc: 0.942 / Cor.coef. Fo:Fc free: 0.946 / SU B: 4.523 / SU ML: 0.121 / Cross valid method: THROUGHOUT / ESU R: 0.191 / ESU R Free: 0.163 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 37.362 Å2
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Refinement step | Cycle: 1 / Resolution: 1.9→33.28 Å
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Refine LS restraints |
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