+Open data
-Basic information
Entry | Database: PDB / ID: 4uj8 | ||||||
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Title | Structure of surface layer protein SbsC, domains 6-7 | ||||||
Components | SURFACE LAYER PROTEIN | ||||||
Keywords | MEMBRANE PROTEIN / SURFACE LAYER / SELF-ASSEMBLY | ||||||
Function / homology | Function and homology information | ||||||
Biological species | GEOBACILLUS STEAROTHERMOPHILUS (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Dordic, A. / Pavkov-Keller, T. / Eder, M. / Egelseer, E.M. / Davis, K. / Mills, D. / Sleytr, U.B. / Kuehlbrandt, W. / Vonck, J. / Keller, W. | ||||||
Citation | Journal: To be Published Title: Structure of Surface Layer Protein Sbsc Authors: Pavkov-Keller, T. / Dordic, A. / Eder, M. / Egelseer, E.M. / Davis, K. / Mills, D. / Sleytr, U.B. / Kuehlbrandt, W. / Vonck, J. / Keller, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4uj8.cif.gz | 219.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4uj8.ent.gz | 175.9 KB | Display | PDB format |
PDBx/mmJSON format | 4uj8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4uj8_validation.pdf.gz | 447.1 KB | Display | wwPDB validaton report |
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Full document | 4uj8_full_validation.pdf.gz | 452.4 KB | Display | |
Data in XML | 4uj8_validation.xml.gz | 49.7 KB | Display | |
Data in CIF | 4uj8_validation.cif.gz | 76.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uj/4uj8 ftp://data.pdbj.org/pub/pdb/validation_reports/uj/4uj8 | HTTPS FTP |
-Related structure data
Related structure data | 5ftxC 4uieS 4uj7S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 24672.604 Da / Num. of mol.: 4 / Fragment: RSBSC, UNP RESDIUES 642-882 Source method: isolated from a genetically manipulated source Source: (gene. exp.) GEOBACILLUS STEAROTHERMOPHILUS (bacteria) Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: O68840 #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.4 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM30A / Wavelength: 0.98 |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→28.6 Å / Num. obs: 93503 / % possible obs: 91.6 % / Observed criterion σ(I): 2.4 / Redundancy: 1.9 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 8.5 |
Reflection shell | Resolution: 1.7→1.8 Å / Redundancy: 1.8 % / Rmerge(I) obs: 0.29 / Mean I/σ(I) obs: 2.4 / % possible all: 91 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRIES 4UJ7, 4UIE Resolution: 1.7→23.001 Å / SU ML: 0.21 / σ(F): 1.99 / Phase error: 24.66 / Stereochemistry target values: MLHL
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→23.001 Å
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Refine LS restraints |
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LS refinement shell |
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