- PDB-3jyc: Crystal structure of the eukaryotic strong inward-rectifier K+ ch... -
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Basic information
Entry
Database: PDB / ID: 3jyc
Title
Crystal structure of the eukaryotic strong inward-rectifier K+ channel Kir2.2 at 3.1 Angstrom resolution
Components
Inward-rectifier K+ channel Kir2.2
Keywords
METAL TRANSPORT / a transmembrane pore and a cytoplasmic pore
Function / homology
Function and homology information
Activation of G protein gated Potassium channels / Classical Kir channels / Phase 4 - resting membrane potential / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / inward rectifier potassium channel activity / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / potassium ion import across plasma membrane / potassium ion transport / protein homotetramerization ...Activation of G protein gated Potassium channels / Classical Kir channels / Phase 4 - resting membrane potential / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / inward rectifier potassium channel activity / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / potassium ion import across plasma membrane / potassium ion transport / protein homotetramerization / identical protein binding / membrane / plasma membrane Similarity search - Function
Mass: 39.098 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: K
Compound details
THE ELECTRON DENSITY FOR RESIDUES 61-69 IS NOT OBSERVED EVEN THOUGH THESE AMINO ACIDS ARE PRESENT ...THE ELECTRON DENSITY FOR RESIDUES 61-69 IS NOT OBSERVED EVEN THOUGH THESE AMINO ACIDS ARE PRESENT IN THE CONSTRUCT. THE PROTEIN IS A TETRAMER WHICH CAN BE GENERATED BY THE CRYSTAL SYMMETRY (DESCRIBED IN REMARK 350) AND THE LACK OF DENSITY FOR RESIDUES 61-69 ALLOWS FOR TWO POSSIBLE CONNECTIVITIES. GIVEN THE DISTANCES RESIDUES 43-60 MOST LIKELY CONNECT TO RESIDUES 70-372 OF A NEIGHBORING SUBUNIT.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.4 Å3/Da / Density % sol: 72.03 %
Crystal grow
Temperature: 277.1 K / Method: vapor diffusion, hanging drop Details: 7-20% PEG 400, 0.5M sodium or potassium chloride, 50mM Buffer, pH 6-9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.1K PH range: 6-9.5
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