+Open data
-Basic information
Entry | Database: PDB / ID: 3spj | ||||||
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Title | Apo inward rectifier potassium channel Kir2.2 I223L mutant | ||||||
Components | Inward-rectifier K+ channel Kir2.2 | ||||||
Keywords | METAL TRANSPORT / PIP / membrane protein / lipid / receptor | ||||||
Function / homology | Function and homology information Activation of G protein gated Potassium channels / Classical Kir channels / Phase 4 - resting membrane potential / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / inward rectifier potassium channel activity / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / potassium ion import across plasma membrane / potassium ion transport / protein homotetramerization ...Activation of G protein gated Potassium channels / Classical Kir channels / Phase 4 - resting membrane potential / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / inward rectifier potassium channel activity / regulation of monoatomic ion transmembrane transport / monoatomic ion channel complex / potassium ion import across plasma membrane / potassium ion transport / protein homotetramerization / membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.307 Å | ||||||
Authors | Hansen, S.B. / Tao, X. / MacKinnon, R. | ||||||
Citation | Journal: Nature / Year: 2011 Title: Structural basis of PIP(2) activation of the classical inward rectifier K(+) channel Kir2.2. Authors: Hansen, S.B. / Tao, X. / Mackinnon, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3spj.cif.gz | 79.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3spj.ent.gz | 57.9 KB | Display | PDB format |
PDBx/mmJSON format | 3spj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sp/3spj ftp://data.pdbj.org/pub/pdb/validation_reports/sp/3spj | HTTPS FTP |
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-Related structure data
Related structure data | 3spcC 3spgC 3sphSC 3spiC C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 39361.723 Da / Num. of mol.: 1 / Mutation: I223L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Gallus gallus (chicken) / Gene: Kir2.2 / Production host: Pichia pastoris (fungus) / References: UniProt: D2YW45, UniProt: F1NHE9*PLUS |
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#2: Chemical | ChemComp-K / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 4.39 Å3/Da / Density % sol: 72 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 10.62% PEG400, 0.5 M sodium chloride, 0.05 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X29A |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 27, 2011 |
Radiation | Monochromator: Rosenbaum-Rock double crystal sagittal focusing monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.3→50 Å / Num. obs: 10028 / % possible obs: 99 % / Observed criterion σ(I): 3 / Redundancy: 7.5 % / Rmerge(I) obs: 0.0118 / Net I/σ(I): 14.5 |
Reflection shell | Resolution: 3.3→3.4 Å / Redundancy: 7.4 % / Rmerge(I) obs: 0.0526 / Mean I/σ(I) obs: 3.7 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 3SPH Resolution: 3.307→28.364 Å / SU ML: 0.36 / σ(F): 0 / Phase error: 28.73 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 97.946 Å2 / ksol: 0.291 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 3.307→28.364 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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