+Open data
-Basic information
Entry | Database: PDB / ID: 2ka4 | ||||||
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Title | NMR structure of the CBP-TAZ1/STAT2-TAD complex | ||||||
Components |
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Keywords | Transcription regulator / CBP/p300 / STAT2 / TAZ1 / transactivation domain / Bromodomain / Activator / Alternative splicing / Antiviral defense / Cytoplasm / DNA-binding / Host-virus interaction / Nucleus / Phosphoprotein / Polymorphism / SH2 domain / Transcription / Transcription regulation | ||||||
Function / homology | Function and homology information ISGF3 complex / Activation of the TFAP2 (AP-2) family of transcription factors / Regulation of FOXO transcriptional activity by acetylation / TRAF6 mediated IRF7 activation / Nuclear events mediated by NFE2L2 / Attenuation phase / Regulation of gene expression by Hypoxia-inducible Factor / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / Transcriptional and post-translational regulation of MITF-M expression and activity / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells ...ISGF3 complex / Activation of the TFAP2 (AP-2) family of transcription factors / Regulation of FOXO transcriptional activity by acetylation / TRAF6 mediated IRF7 activation / Nuclear events mediated by NFE2L2 / Attenuation phase / Regulation of gene expression by Hypoxia-inducible Factor / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / Transcriptional and post-translational regulation of MITF-M expression and activity / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / cAMP response element binding protein binding / Formation of the beta-catenin:TCF transactivating complex / NOTCH1 Intracellular Domain Regulates Transcription / RUNX3 regulates NOTCH signaling / Notch-HLH transcription pathway / positive regulation of cell adhesion molecule production / germ-line stem cell population maintenance / negative regulation of viral process / Regulation of lipid metabolism by PPARalpha / Cytoprotection by HMOX1 / CD209 (DC-SIGN) signaling / Estrogen-dependent gene expression / peptide lactyltransferase (CoA-dependent) activity / outer kinetochore / negative regulation of interferon-beta production / histone H3K18 acetyltransferase activity / N-terminal peptidyl-lysine acetylation / histone H3K27 acetyltransferase activity / MRF binding / peroxisome proliferator activated receptor binding / face morphogenesis / negative regulation of transcription by RNA polymerase I / peptide-lysine-N-acetyltransferase activity / negative regulation of type I interferon-mediated signaling pathway / cellular response to hepatocyte growth factor stimulus / positive regulation of G1/S transition of mitotic cell cycle / Interleukin-20 family signaling / type I interferon-mediated signaling pathway / positive regulation of dendritic spine development / regulation of mitochondrial fission / ubiquitin-like protein ligase binding / SMAD binding / acetyltransferase activity / cell surface receptor signaling pathway via JAK-STAT / behavioral response to cocaine / TFIIB-class transcription factor binding / Regulation of IFNA/IFNB signaling / histone acetyltransferase complex / positive regulation of double-strand break repair via homologous recombination / long-term memory / histone acetyltransferase activity / histone acetyltransferase / transcription coregulator activity / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / RNA polymerase II transcription regulatory region sequence-specific DNA binding / regulation of protein phosphorylation / positive regulation of DNA-binding transcription factor activity / protein destabilization / protein modification process / positive regulation of non-canonical NF-kappaB signal transduction / PML body / Evasion by RSV of host interferon responses / chromatin DNA binding / RNA polymerase II transcription regulator complex / cellular response to virus / response to peptide hormone / transcription coactivator binding / defense response / rhythmic process / disordered domain specific binding / cellular response to UV / Interferon alpha/beta signaling / regulation of cell population proliferation / DNA-binding transcription factor binding / Potential therapeutics for SARS / transcription regulator complex / defense response to virus / transcription coactivator activity / RNA polymerase II-specific DNA-binding transcription factor binding / damaged DNA binding / molecular adaptor activity / DNA-binding transcription factor activity, RNA polymerase II-specific / nuclear body / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein domain specific binding / DNA-binding transcription factor activity / chromatin binding / regulation of transcription by RNA polymerase II / positive regulation of gene expression / positive regulation of DNA-templated transcription / chromatin / regulation of DNA-templated transcription / protein-containing complex binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / zinc ion binding / nucleoplasm / identical protein binding Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) Homo sapiens (human) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Wojciak, J.M. / Martinez-Yamout, M.A. / Dyson, H.J. / Wright, P.E. | ||||||
Citation | Journal: Embo J. / Year: 2009 Title: Structural basis for recruitment of CBP/p300 coactivators by STAT1 and STAT2 transactivation domains Authors: Wojciak, J.M. / Martinez-Yamout, M.A. / Dyson, H.J. / Wright, P.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ka4.cif.gz | 961.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ka4.ent.gz | 813.6 KB | Display | PDB format |
PDBx/mmJSON format | 2ka4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2ka4_validation.pdf.gz | 423.4 KB | Display | wwPDB validaton report |
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Full document | 2ka4_full_validation.pdf.gz | 653 KB | Display | |
Data in XML | 2ka4_validation.xml.gz | 62.2 KB | Display | |
Data in CIF | 2ka4_validation.cif.gz | 82.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ka/2ka4 ftp://data.pdbj.org/pub/pdb/validation_reports/ka/2ka4 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11281.177 Da / Num. of mol.: 1 / Fragment: UNP residues 340 to 439 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Species: musculus / Gene: Crebbp / Species (production host): coli / Production host: Escherichia coli (E. coli) / References: UniProt: Q6GQV9, UniProt: P45481*PLUS |
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#2: Protein | Mass: 6430.196 Da / Num. of mol.: 1 / Fragment: UNP residues 786 to 838 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Species: sapiens / Gene: STAT2 / Species (production host): coli / Production host: Escherichia coli (E. coli) / References: UniProt: P52630 |
#3: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D 1H-15N HSQC |
-Sample preparation
Details | Contents: 1 mM [U-100% 15N] ALANINE, 93% H2O/7% D2O / Solvent system: 93% H2O/7% D2O |
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Sample | Conc.: 1 mM / Component: ALANINE / Isotopic labeling: [U-100% 15N] |
Sample conditions | Ionic strength: 0.05 / pH: 6.8 / Pressure: ambient / Temperature: 305 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: Amber Developer: Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm Classification: refinement |
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Refinement | Method: molecular dynamics / Software ordinal: 1 |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |