+Open data
-Basic information
Entry | Database: PDB / ID: 2fju | ||||||
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Title | Activated Rac1 bound to its effector phospholipase C beta 2 | ||||||
Components |
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Keywords | SIGNALING PROTEIN / APOPTOSIS/HYDROLASE / Protein-protein complex / APOPTOSIS-HYDROLASE COMPLEX | ||||||
Function / homology | Function and homology information G-protein beta/gamma-subunit complex / phosphoinositide phospholipase C / Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / Acetylcholine regulates insulin secretion / regulation of respiratory burst / negative regulation of interleukin-23 production / regulation of neutrophil migration / localization within membrane / Activated NTRK2 signals through CDK5 / detection of chemical stimulus involved in sensory perception of bitter taste ...G-protein beta/gamma-subunit complex / phosphoinositide phospholipase C / Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / Acetylcholine regulates insulin secretion / regulation of respiratory burst / negative regulation of interleukin-23 production / regulation of neutrophil migration / localization within membrane / Activated NTRK2 signals through CDK5 / detection of chemical stimulus involved in sensory perception of bitter taste / negative regulation of receptor-mediated endocytosis / regulation of hydrogen peroxide metabolic process / phosphatidylinositol metabolic process / PLC beta mediated events / ruffle assembly / engulfment of apoptotic cell / NTRK2 activates RAC1 / Inactivation of CDC42 and RAC1 / NADPH oxidase complex / respiratory burst / WNT5:FZD7-mediated leishmania damping / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / cortical cytoskeleton organization / phosphatidylinositol phospholipase C activity / hepatocyte growth factor receptor signaling pathway / phospholipase C activity / ruffle organization / cell projection assembly / thioesterase binding / regulation of stress fiber assembly / negative regulation of fibroblast migration / RHO GTPases activate CIT / sphingosine-1-phosphate receptor signaling pathway / Nef and signal transduction / motor neuron axon guidance / PCP/CE pathway / positive regulation of neutrophil chemotaxis / regulation of nitric oxide biosynthetic process / RHO GTPases activate KTN1 / Activation of RAC1 / : / regulation of lamellipodium assembly / Azathioprine ADME / MET activates RAP1 and RAC1 / DCC mediated attractive signaling / positive regulation of cell-substrate adhesion / Wnt signaling pathway, planar cell polarity pathway / Sema4D mediated inhibition of cell attachment and migration / CD28 dependent Vav1 pathway / Ephrin signaling / lamellipodium assembly / positive regulation of Rho protein signal transduction / regulation of cell size / establishment or maintenance of cell polarity / Activation of RAC1 downstream of NMDARs / small GTPase-mediated signal transduction / Rho GDP-dissociation inhibitor binding / NRAGE signals death through JNK / Rac protein signal transduction / Synthesis of IP3 and IP4 in the cytosol / neuronal dense core vesicle / RHO GTPases activate PAKs / positive regulation of focal adhesion assembly / phosphatidylinositol-mediated signaling / semaphorin-plexin signaling pathway / Sema3A PAK dependent Axon repulsion / ficolin-1-rich granule membrane / RHO GTPases Activate NADPH Oxidases / EPH-ephrin mediated repulsion of cells / anatomical structure morphogenesis / RHO GTPases Activate WASPs and WAVEs / RHO GTPases activate IQGAPs / phospholipid metabolic process / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / positive regulation of lamellipodium assembly / lipid catabolic process / positive regulation of substrate adhesion-dependent cell spreading / release of sequestered calcium ion into cytosol / RHO GTPases activate PKNs / Signal transduction by L1 / positive regulation of stress fiber assembly / GPVI-mediated activation cascade / positive regulation of microtubule polymerization / EPHB-mediated forward signaling / RAC1 GTPase cycle / regulation of cell migration / actin filament polymerization / positive regulation of endothelial cell migration / substrate adhesion-dependent cell spreading / cell chemotaxis / cell-matrix adhesion / small monomeric GTPase / secretory granule membrane / VEGFR2 mediated vascular permeability / G protein activity / actin filament organization / Translocation of SLC2A4 (GLUT4) to the plasma membrane / cell motility / RHO GTPases Activate Formins / regulation of actin cytoskeleton organization Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR, MAD, molecular replacement / Resolution: 2.2 Å | ||||||
Authors | Jezyk, M.R. / Snyder, J.T. / Harden, T.K. / Sondek, J. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2006 Title: Crystal structure of Rac1 bound to its effector phospholipase C-beta2. Authors: Jezyk, M.R. / Snyder, J.T. / Gershberg, S. / Worthylake, D.K. / Harden, T.K. / Sondek, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fju.cif.gz | 201.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fju.ent.gz | 156.4 KB | Display | PDB format |
PDBx/mmJSON format | 2fju.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2fju_validation.pdf.gz | 786.4 KB | Display | wwPDB validaton report |
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Full document | 2fju_full_validation.pdf.gz | 806.9 KB | Display | |
Data in XML | 2fju_validation.xml.gz | 39.1 KB | Display | |
Data in CIF | 2fju_validation.cif.gz | 57.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fj/2fju ftp://data.pdbj.org/pub/pdb/validation_reports/fj/2fju | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 19797.844 Da / Num. of mol.: 1 / Fragment: residues 1-189 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAC1 / Production host: Escherichia coli (E. coli) / References: UniProt: P63000 |
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#2: Protein | Mass: 91033.922 Da / Num. of mol.: 1 / Fragment: residues 1-799 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PLCB2 / Plasmid: pVL1392 / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): Sf21 References: UniProt: Q00722, phosphoinositide phospholipase C |
-Non-polymers , 4 types, 489 molecules
#3: Chemical | ChemComp-MG / |
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#4: Chemical | ChemComp-GSP / |
#5: Chemical | ChemComp-CA / |
#6: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.22 Å3/Da / Density % sol: 70.83 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 8% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 1.009 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 4, 2004 |
Radiation | Monochromator: Si 220 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.009 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→15 Å / Num. all: 94114 / Num. obs: 93710 |
Reflection shell | Resolution: 2.2→2.28 Å |
-Processing
Software |
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Refinement | Method to determine structure: MIR, MAD, molecular replacement Resolution: 2.2→15 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 32.547 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→15 Å
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