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Yorodumi- PDB-1kvn: Solution Structure Of Protein SRP19 Of The Arhaeoglobus fulgidus ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1kvn | ||||||
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Title | Solution Structure Of Protein SRP19 Of The Arhaeoglobus fulgidus Signal Recognition Particle, 10 Structures | ||||||
Components | SRP19 | ||||||
Keywords | RNA BINDING PROTEIN | ||||||
Function / homology | Function and homology information SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition / signal recognition particle / 7S RNA binding Similarity search - Function | ||||||
Biological species | Archaeoglobus fulgidus (archaea) | ||||||
Method | SOLUTION NMR / Distance geometry, simulated annealing protocol | ||||||
Authors | Pakhomova, O.N. / Deep, S. / Huang, Q. / Zwieb, C. / Hinck, A.P. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2002 Title: Solution structure of protein SRP19 of Archaeoglobus fulgidus signal recognition particle. Authors: Pakhomova, O.N. / Deep, S. / Huang, Q. / Zwieb, C. / Hinck, A.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1kvn.cif.gz | 358.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1kvn.ent.gz | 296.5 KB | Display | PDB format |
PDBx/mmJSON format | 1kvn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1kvn_validation.pdf.gz | 345.3 KB | Display | wwPDB validaton report |
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Full document | 1kvn_full_validation.pdf.gz | 445.4 KB | Display | |
Data in XML | 1kvn_validation.xml.gz | 25.8 KB | Display | |
Data in CIF | 1kvn_validation.cif.gz | 38.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kv/1kvn ftp://data.pdbj.org/pub/pdb/validation_reports/kv/1kvn | HTTPS FTP |
-Related structure data
Related structure data | 1kvvC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12406.707 Da / Num. of mol.: 1 / Mutation: C4S/C41S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Archaeoglobus fulgidus (archaea) / Gene: AF1258 / Plasmid: pET-23c(+) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O29010 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: IPAP-HSQC experiment was performed in a sample of the protein in either an unstressed or mechanically stressed 8% polyacrylamide gel. |
-Sample preparation
Details |
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Sample conditions |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AMX2 / Manufacturer: Bruker / Model: AMX2 / Field strength: 500 MHz |
-Processing
NMR software |
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Refinement | Method: Distance geometry, simulated annealing protocol / Software ordinal: 1 Details: The structures are based on a total of 886 restraints, 690 are NOE-derived distance constraints, 130 dihedral angle restraints, 66 1H-15N residual dipolar coupling restraints. | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 26 / Conformers submitted total number: 10 |