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Yorodumi- PDB-1klp: The Solution Structure of Acyl Carrier Protein from Mycobacterium... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1klp | ||||||
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Title | The Solution Structure of Acyl Carrier Protein from Mycobacterium tuberculosis | ||||||
Components | MEROMYCOLATE EXTENSION ACYL CARRIER PROTEIN | ||||||
Keywords | LIGAND TRANSPORT / four-helix bundle | ||||||
Function / homology | Function and homology information acyl-CoA metabolic process / cell wall / lipid A biosynthetic process / acyl binding / acyl carrier activity / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Mycobacterium tuberculosis (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics simulated annealing | ||||||
Authors | Wong, H.C. / Liu, G. / Zhang, Y.-M. / Rock, C.O. / Zheng, J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2002 Title: The solution structure of acyl carrier protein from Mycobacterium tuberculosis. Authors: Wong, H.C. / Liu, G. / Zhang, Y.M. / Rock, C.O. / Zheng, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1klp.cif.gz | 632.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1klp.ent.gz | 524.8 KB | Display | PDB format |
PDBx/mmJSON format | 1klp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1klp_validation.pdf.gz | 342 KB | Display | wwPDB validaton report |
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Full document | 1klp_full_validation.pdf.gz | 490.9 KB | Display | |
Data in XML | 1klp_validation.xml.gz | 64.4 KB | Display | |
Data in CIF | 1klp_validation.cif.gz | 82.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kl/1klp ftp://data.pdbj.org/pub/pdb/validation_reports/kl/1klp | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12532.871 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Strain: H37Rv / Gene: acpM / Variant: H37Rv / Plasmid: pET11a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 CodonPlus (DE3)-RP / References: UniProt: P0A4W6, UniProt: P9WQF3*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using triple-resonance NMR spectroscopy. |
-Sample preparation
Details | Contents: 2 mM 15N,13C; 40 mM potassium phosphate pH 6.5; 0.1 mM DTT; 0.1% NaN3 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 0.1 / pH: 6.5 / Pressure: ambient / Temperature: 300 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics simulated annealing / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest target function | ||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 320 / Conformers submitted total number: 20 |