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Yorodumi- PDB-1h3z: Solution structure of a PWWP domain from Schizosaccharomyces Pombe -
+Open data
-Basic information
Entry | Database: PDB / ID: 1h3z | ||||||
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Title | Solution structure of a PWWP domain from Schizosaccharomyces Pombe | ||||||
Components | HYPOTHETICAL 62.8 KDA PROTEIN C215.07C | ||||||
Keywords | NUCLEAR PROTEIN / PWWP / CHROMATIN / BETA-BARREL | ||||||
Function / homology | Function and homology information chromatin => GO:0000785 / methylated histone binding / chromatin remodeling / regulation of transcription by RNA polymerase II / nucleus Similarity search - Function | ||||||
Biological species | SCHIZOSACCHAROMYCES POMBE (fission yeast) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY SIMULATED ANNEALING | ||||||
Authors | Slater, L.M. / Allen, M.D. / Bycroft, M. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: Structural Variation in Pwwp Domains Authors: Slater, L.M. / Allen, M.D. / Bycroft, M. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1h3z.cif.gz | 762.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1h3z.ent.gz | 662.8 KB | Display | PDB format |
PDBx/mmJSON format | 1h3z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h3/1h3z ftp://data.pdbj.org/pub/pdb/validation_reports/h3/1h3z | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12210.151 Da / Num. of mol.: 1 / Fragment: PWWP DOMAIN, RESIDUES 118-225 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SCHIZOSACCHAROMYCES POMBE (fission yeast) Description: SYNTHETIC GENE / Plasmid: PRSETA-GROEL / Production host: ESCHERICHIA COLI BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: O94312 |
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Compound details | THE PWWP DOMAIN IS NAMED AFTER A CONSERVED PRO-TRP-TRP-PRO MOTIF. IT IS PRESENT IN PROTEINS OF ...THE PWWP DOMAIN IS NAMED AFTER A CONSERVED PRO-TRP-TRP-PRO MOTIF. IT IS PRESENT IN PROTEINS OF NUCLEAR ORIGIN AND PLAYS A ROLE IN CEL GROWTH AND DIFFERENTI |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: STANDARD |
NMR details | Text: THE STRUCTURE WAS ASSIGNED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELLED PWWP. |
-Sample preparation
Sample conditions | Ionic strength: 0.2 / pH: 6.5 / Temperature: 290 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: DISTANCE GEOMETRY SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | ||||||||||||
NMR ensemble | Conformer selection criteria: NO VIOLATION GREATER THAN 0.25A AND NO VIOLATIONS GREATER THAN 5 DEGREES Conformers calculated total number: 30 / Conformers submitted total number: 23 |