+Open data
-Basic information
Entry | Database: PDB / ID: 1ggw | ||||||
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Title | CDC4P FROM SCHIZOSACCHAROMYCES POMBE | ||||||
Components | PROTEIN (CDC4P) | ||||||
Keywords | CYTOKINE / LIGHT CHAIN / CYTOKINESIS / CELL CYCLE / EF-HAND | ||||||
Function / homology | Function and homology information protein localization to medial cortex / RHO GTPases activate PAKs / medial cortical node / mitotic actomyosin contractile ring assembly actin filament organization / mitotic actomyosin contractile ring / mitotic actomyosin contractile ring assembly / myosin II complex / division septum assembly / cell division site / metal ion binding ...protein localization to medial cortex / RHO GTPases activate PAKs / medial cortical node / mitotic actomyosin contractile ring assembly actin filament organization / mitotic actomyosin contractile ring / mitotic actomyosin contractile ring assembly / myosin II complex / division septum assembly / cell division site / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Schizosaccharomyces pombe (fission yeast) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Slupsky, C.M. / Hemmingsen, S.M. / McIntosh, L.P. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2001 Title: Structure of Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe. Authors: Slupsky, C.M. / Desautels, M. / Huebert, T. / Zhao, R. / Hemmingsen, S.M. / McIntosh, L.P. #1: Journal: J.Biol.Chem. / Year: 2001 Title: Cdc4p, a Contractile Ring Protein Essential for Cytokinesis in Schizosaccharomyces pombe, Interacts with a Phosphatidylinositol 4-Kinase Authors: Desautels, M. / Den Haese, J.P. / Slupsky, C.M. / McIntosh, L.P. / Hemmingsen, S.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ggw.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb1ggw.ent.gz | 908.4 KB | Display | PDB format |
PDBx/mmJSON format | 1ggw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gg/1ggw ftp://data.pdbj.org/pub/pdb/validation_reports/gg/1ggw | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15543.479 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Schizosaccharomyces pombe (fission yeast) Strain: WILD-TYPE 972 / Cellular location: CYTOPLASM / Plasmid: PT7-7 / Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Gene (production host): CDC4 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q09196 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C,15N-LABELED CDC4P, AND 15N-LABELED CDC4P |
-Sample preparation
Details | Contents: H2O / D2O |
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Sample conditions | Ionic strength: 100 mM KCL / pH: 6.5 / Pressure: 1.0 ATM / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JOURNAL CITATION ABOVE | ||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 50 / Conformers submitted total number: 26 |