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Yorodumi- PDB-1dg9: CRYSTAL STRUCTURE OF BOVINE LOW MOLECULAR WEIGHT PTPASE COMPLEXED... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1dg9 | ||||||
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Title | CRYSTAL STRUCTURE OF BOVINE LOW MOLECULAR WEIGHT PTPASE COMPLEXED WITH HEPES | ||||||
Components | TYROSINE PHOSPHATASE | ||||||
Keywords | HYDROLASE / PTPASE / HEPES COMPLEX | ||||||
Function / homology | Function and homology information acid phosphatase / acid phosphatase activity / non-membrane spanning protein tyrosine phosphatase activity / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity / cytoplasm Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.9 Å | ||||||
Authors | Zhang, M. / Zhou, M. / Van Etten, R.L. / Stauffacher, C.V. | ||||||
Citation | Journal: Biochemistry / Year: 1997 Title: Crystal structure of bovine low molecular weight phosphotyrosyl phosphatase complexed with the transition state analog vanadate. Authors: Zhang, M. / Zhou, M. / Van Etten, R.L. / Stauffacher, C.V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1dg9.cif.gz | 42.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1dg9.ent.gz | 29.7 KB | Display | PDB format |
PDBx/mmJSON format | 1dg9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1dg9_validation.pdf.gz | 381.3 KB | Display | wwPDB validaton report |
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Full document | 1dg9_full_validation.pdf.gz | 387.2 KB | Display | |
Data in XML | 1dg9_validation.xml.gz | 5.8 KB | Display | |
Data in CIF | 1dg9_validation.cif.gz | 7.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dg/1dg9 ftp://data.pdbj.org/pub/pdb/validation_reports/dg/1dg9 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 17946.326 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Organ: HEART / Plasmid: PET / Production host: Escherichia coli (E. coli) / References: UniProt: P11064, protein-tyrosine-phosphatase |
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#2: Chemical | ChemComp-EPE / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.54 % | ||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion / pH: 7.5 Details: 20% PEG4k, 10% 2-propanol, 100mM HEPES, pH 7.5, VAPOR DIFFUSION Temp details: RT | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Jan 1, 1996 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.9 Å / Num. obs: 71205 / % possible obs: 98.3 % / Rmerge(I) obs: 0.065 |
Reflection shell | Highest resolution: 1.9 Å |
Reflection | *PLUS Num. obs: 12122 / Num. measured all: 71205 |
-Processing
Software |
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Refinement | Resolution: 1.9→20 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: engh & huber /
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Refinement step | Cycle: LAST / Resolution: 1.9→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||
Refine LS restraints | *PLUS
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