+Open data
-Basic information
Entry | Database: PDB / ID: 1b26 | ||||||
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Title | GLUTAMATE DEHYDROGENASE | ||||||
Components | GLUTAMATE DEHYDROGENASE | ||||||
Keywords | OXIDOREDUCTASE / METABOLIC ROLE / GLUTAMATE / DEHYDROGENASE | ||||||
Function / homology | Function and homology information glutamate dehydrogenase [NAD(P)+] / glutamate dehydrogenase (NADP+) activity / glutamate dehydrogenase (NAD+) activity / glutamate catabolic process Similarity search - Function | ||||||
Biological species | Thermotoga maritima (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 3 Å | ||||||
Authors | Knapp, S. / Devos, W.M. / Rice, D. / Ladenstein, R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1997 Title: Crystal structure of glutamate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima at 3.0 A resolution. Authors: Knapp, S. / de Vos, W.M. / Rice, D. / Ladenstein, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1b26.cif.gz | 415.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1b26.ent.gz | 350.8 KB | Display | PDB format |
PDBx/mmJSON format | 1b26.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1b26_validation.pdf.gz | 448.1 KB | Display | wwPDB validaton report |
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Full document | 1b26_full_validation.pdf.gz | 477.7 KB | Display | |
Data in XML | 1b26_validation.xml.gz | 79.8 KB | Display | |
Data in CIF | 1b26_validation.cif.gz | 107.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b2/1b26 ftp://data.pdbj.org/pub/pdb/validation_reports/b2/1b26 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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-Components
#1: Protein | Mass: 45859.473 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermotoga maritima (bacteria) / Cellular location: CYTOPLASM / Species (production host): Escherichia coli / Cellular location (production host): CYTOPLASM / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 DE3 References: UniProt: P96110, glutamate dehydrogenase [NAD(P)+] |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 60.4 % | ||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: CRYSTALLIZATION CONDITIONS: 3% POLYETHYLENE GLYCOLE, 120 MM AMMONIUM ACETATE 50 MM BIS-TRIS PROPANE PH 6.5 | ||||||||||||||||||||
Crystal grow | *PLUS Method: unknown | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 286 K |
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Diffraction source | Source: ROTATING ANODE / Type: MACSCIENCE / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 3→20 Å / Num. obs: 57305 / % possible obs: 94 % / Redundancy: 3.9 % / Rmerge(I) obs: 0.091 / Rsym value: 0.048 |
Reflection shell | Resolution: 3→3.1 Å / % possible all: 75 |
Reflection | *PLUS Num. measured all: 220980 |
Reflection shell | *PLUS % possible obs: 75 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3→8 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Displacement parameters | Biso mean: 40.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→8 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file | Serial no: 1 / Param file: PARAM19X.PRO / Topol file: TOPH19X.PRO | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: X-PLOR / Version: 3.8 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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