rRNA cytidine N-acetyltransferase activity / tRNA acetylation / mRNA modification / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex ...rRNA cytidine N-acetyltransferase activity / tRNA acetylation / mRNA modification / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex / snRNA binding / rRNA base methylation / protein localization to nucleolus / U3 snoRNA binding / rRNA export from nucleus / sno(s)RNA-containing ribonucleoprotein complex / 90S preribosome / preribosome, small subunit precursor / snoRNA binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 転写後修飾 / small-subunit processome / ribosomal small subunit biogenesis / nuclear periphery / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / 助触媒 / RNA splicing / small ribosomal subunit rRNA binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA processing / 転写後修飾 / methyltransferase activity / リボソーム生合成 / cytosolic small ribosomal subunit / ribonucleoprotein complex / small ribosomal subunit / nucleic acid binding / tRNA binding / メチル化 / rRNA binding / リボソーム / structural constituent of ribosome / protein ubiquitination / 翻訳 (生物学) / GTPase activity / GTP binding / 核小体 / RNA binding / 生体膜 / ATP binding / identical protein binding / 細胞核 / 細胞質
Ribosomal protein L1p/L10e family / WD40-repeat-containing domain superfamily / 50S ribosomal protein L30e-like / Bms1/Tsr1-type G domain / TmcA/NAT10/Kre33 / RNA cytidine acetyltransferase NAT10 / Nrap protein domain 1 / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 ...Ribosomal protein L1p/L10e family / WD40-repeat-containing domain superfamily / 50S ribosomal protein L30e-like / Bms1/Tsr1-type G domain / TmcA/NAT10/Kre33 / RNA cytidine acetyltransferase NAT10 / Nrap protein domain 1 / Nrap protein, domain 2 / Nrap protein, domain 3 / Nrap protein, domain 4 / Nrap protein, domain 5 / Nrap protein, domain 6 / Ribosomal protein S8 superfamily / Nop domain superfamily / RNA 3'-terminal phosphate cyclase, insert domain superfamily / Alpha/beta knot methyltransferases / K Homology domain, type 1 superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein S11 superfamily / RNA-binding S4 domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / Ribosome biogenesis protein Bms1, N-terminal / Ribosomal RNA-processing protein Rrp9-like / Ribosome biogenesis protein Bms1/Tsr1 / Fcf2/DNTTIP2 / Pre-rRNA-processing protein PNO1-like / U3 small nucleolar RNA-associated protein 10 / WD repeat-containing protein WDR46/Utp7 / S-adenosyl-L-methionine-dependent methyltransferase / tRNA (guanine-N1-)-methyltransferase, N-terminal / Rrp7, RRM-like N-terminal domain / Ribosomal protein S4/S9 / Pyrrolo-quinoline quinone beta-propeller repeat / Ribosomal protein L7Ae/L8/Nhp2 family / Sas10 C-terminal domain / U3 small nucleolar RNA-associated protein 15, C-terminal / Tetratricopeptide repeat / WD40 repeat, conserved site / G-protein beta WD-40 repeat / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S9, conserved site / Ribosomal protein S7, conserved site / Fibrillarin, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / RNA-binding domain, S1 / Helix hairpin bin domain superfamily / Ribosomal protein S15P / Ribosomal protein L1-like / RNA 3'-terminal phosphate cyclase domain / Ribosomal protein S7 domain / Ribosomal RNA assembly KRR1 / Ribosomal RNA-processing protein 7, C-terminal domain / Periodic tryptophan protein 2 / Nucleolar complex protein 4 / P-loop containing nucleoside triphosphate hydrolase / Possible tRNA binding domain / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein S28e conserved site / Ribosomal RNA-processing protein 7 / Krr1, KH1 domain / Ribosomal S11, conserved site / U3 small nucleolar RNA-associated protein 10 / Ribosomal protein S13/S18 / Ribosomal protein S8 / Ribosomal protein S12/S23 / Ribosomal protein S28e / S1 RNA binding domain / Dip2/Utp12 Family / Nop14-like family / Sas10/Utp3/C1D family / Sas10 C-terminal domain / U3 small nucleolar RNA-associated protein 6 / BING4CT (NUC141) domain / BP28CT (NUC211) domain / Utp11 protein / Ribosomal protein S11 / Utp13 specific WD40 associated domain / Utp14 protein / UTP15 C terminal / U3 snoRNA associated / CBF/Mak21 family / Utp21 specific WD40 associated putative domain / Nrap protein domain 1 / Nrap protein PAP/OAS-like domain / Nrap protein domain 3 / Nrap protein nucleotidyltransferase domain 4 / Nrap protein PAP/OAS1-like domain 5 / Nrap protein domain 6 / Ribosomal protein S9/S16 / Ribosomal S13/S15 N-terminal domain / Nop, C-terminal domain / Brix domain / Nucleotidyltransferase superfamily / Fibrillarin / snoRNA binding domain, fibrillarin / NOP5NT (NUC127) domain / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / WD domain, G-beta repeat / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase (RTC), insert domain
WD_REPEATS_REGION domain-containing protein / CBF domain-containing protein / RNA cytidine acetyltransferase / 40S ribosomal protein S13-like protein / Nucleolar protein 58 / WD_REPEATS_REGION domain-containing protein / RNA 3'-terminal phosphate cyclase-like protein / Utp12 domain-containing protein / DDB1- and CUL4-associated factor 13 / 40S ribosomal protein s23-like protein ...WD_REPEATS_REGION domain-containing protein / CBF domain-containing protein / RNA cytidine acetyltransferase / 40S ribosomal protein S13-like protein / Nucleolar protein 58 / WD_REPEATS_REGION domain-containing protein / RNA 3'-terminal phosphate cyclase-like protein / Utp12 domain-containing protein / DDB1- and CUL4-associated factor 13 / 40S ribosomal protein s23-like protein / 40S ribosomal protein s9-like protein / 40S ribosomal protein s5-like protein / Uncharacterized protein / Uncharacterized protein / U3 small nucleolar RNA-associated protein 22 / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S14-like protein / Periodic tryptophan protein 2-like protein / U3 small nucleolar RNA-associated protein 11 / WD_REPEATS_REGION domain-containing protein / Putative U3 small nucleolar ribonucleoprotein / S4 RNA-binding domain-containing protein / Ribonucloprotein / Uncharacterized protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / 40S ribosomal protein S16-like protein / WD_REPEATS_REGION domain-containing protein / Uncharacterized protein / 40S ribosomal protein S28-like protein / WD_REPEATS_REGION domain-containing protein / Histone-glutamine methyltransferase / Nucleolar protein 58 / Uncharacterized protein / Putative U3 small nucleolar ribonucleoprotein protein / Pre-rRNA-processing protein PNO1 / KRR1 small subunit processome component / Bms1-type G domain-containing protein / Fcf2 domain-containing protein / Sas10 domain-containing protein / Uncharacterized protein / 40S ribosomal protein S22-like protein / Uncharacterized protein / Putative ribosomal protein / Uncharacterized protein / Uncharacterized protein / Putative U3 snoRNP protein / 40S ribosomal protein S7 / Uncharacterized protein CTHT_0071780 / U3 small nucleolar RNA-associated protein 10
ジャーナル: Mol Cell / 年: 2019 タイトル: Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration. 著者: Jingdong Cheng / Jochen Baßler / Paulina Fischer / Benjamin Lau / Nikola Kellner / Ruth Kunze / Sabine Griesel / Martina Kallas / Otto Berninghausen / Daniela Strauss / Roland Beckmann / Ed Hurt / 要旨: Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome ...Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.