+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2k9r | ||||||
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タイトル | Enhancing the activity of insulin by stereospecific unfolding | ||||||
要素 | (Insulinインスリン) x 2 | ||||||
キーワード | HORMONE (ホルモン) / INSULIN (インスリン) / MUTANT (ミュータント (人類)) / Carbohydrate metabolism (炭水化物代謝) / Cleavage on pair of basic residues / Diabetes mellitus (糖尿病) / Disease mutation / Glucose metabolism (炭水化物代謝) / Pharmaceutical (医薬品) / Secreted (分泌) | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / Signaling by Insulin receptor / IRS activation / nitric oxide-cGMP-mediated signaling / negative regulation of fatty acid metabolic process / Insulin processing / negative regulation of feeding behavior / regulation of protein secretion / positive regulation of peptide hormone secretion / Regulation of gene expression in beta cells / positive regulation of respiratory burst / positive regulation of dendritic spine maintenance / alpha-beta T cell activation / negative regulation of acute inflammatory response / negative regulation of respiratory burst involved in inflammatory response / negative regulation of protein secretion / fatty acid homeostasis / Synthesis, secretion, and deacylation of Ghrelin / positive regulation of glycogen biosynthetic process / positive regulation of lipid biosynthetic process / Signal attenuation / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of gluconeogenesis / positive regulation of nitric oxide mediated signal transduction / regulation of protein localization to plasma membrane / COPI-mediated anterograde transport / negative regulation of lipid catabolic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / positive regulation of insulin receptor signaling pathway / negative regulation of reactive oxygen species biosynthetic process / 小胞 / positive regulation of protein autophosphorylation / Insulin receptor recycling / insulin-like growth factor receptor binding / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / neuron projection maintenance / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of brown fat cell differentiation / activation of protein kinase B activity / positive regulation of glycolytic process / Insulin receptor signalling cascade / positive regulation of mitotic nuclear division / Regulation of insulin secretion / positive regulation of nitric-oxide synthase activity / positive regulation of long-term synaptic potentiation / endosome lumen / positive regulation of cytokine production / acute-phase response / positive regulation of protein secretion / regulation of transmembrane transporter activity / positive regulation of cell differentiation / positive regulation of glucose import / negative regulation of proteolysis / regulation of synaptic plasticity / wound healing / insulin receptor binding / negative regulation of protein catabolic process / positive regulation of neuron projection development / hormone activity / 認識 / Golgi lumen / vasodilation / positive regulation of protein localization to nucleus / glucose metabolic process / regulation of protein localization / glucose homeostasis / cell-cell signaling / insulin receptor signaling pathway / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / 小胞体 / ゴルジ体 / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
データ登録者 | Hua, Q.X. / Xu, B. / Huang, K. / Hu, S.Q. / Nakarawa, S. / Jia, W.H. / Philips, N.F.P. / Wittaker, L. / Wittaker, J. / Katsoyannis, P.G. / Weiss, M.A. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2009 タイトル: Enhancing the Activity of a Protein by Stereospecific Unfolding: CONFORMATIONAL LIFE CYCLE OF INSULIN AND ITS EVOLUTIONARY ORIGINS. 著者: Hua, Q.X. / Xu, B. / Huang, K. / Hu, S.Q. / Nakagawa, S. / Jia, W. / Wang, S. / Whittaker, J. / Katsoyannis, P.G. / Weiss, M.A. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2k9r.cif.gz | 301.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2k9r.ent.gz | 260.7 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2k9r.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/k9/2k9r ftp://data.pdbj.org/pub/pdb/validation_reports/k9/2k9r | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 2383.698 Da / 分子数: 1 / 断片: Insulin A chain / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: INS / プラスミド: pBR322 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P01308 |
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#2: タンパク質・ペプチド | 分子量: 3334.798 Da / 分子数: 1 / 断片: Insulin B chain / Mutation: H10D, F24A, P28K, K29P / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: INS / プラスミド: pBR322 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P01308 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: THIS STRUCTURE WAS DETERMINED USING STANDARD 2D HOMONUCLEAR NMR TECHNIQUES. |
-試料調製
詳細 | 内容: 0.5-0.8 mM INSULIN A CHAIN, 0.5-0.8 mM INSULIN B CHAIN, 90% H2O/10% D2O 溶媒系: 90% H2O/10% D2O | ||||||||||||
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試料 |
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試料状態 | イオン強度: 0 / pH: 7.4 / 圧: AMBIENT / 温度: 298 K |
-NMR測定
NMRスペクトロメーター | タイプ: VARIAN INOVA / 製造業者: Varian / モデル: INOVA / 磁場強度: 600 MHz |
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-解析
NMR software |
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精密化 | 手法: DISTANCE GEOMETRY, SIMULATED ANNEALING / ソフトェア番号: 1 詳細: RMSD VALUES FOR ALL 20 STRUCTURES VERSUS GEOMETRIC AVERAGE: (BACKBONE, A2-A19, B3-B26) 0.37 ANGSTR | ||||||||||||
代表構造 | 選択基準: closest to the average | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 40 / 登録したコンフォーマーの数: 20 |