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- PDB-2wnm: Solution structure of Gp2 -

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Basic information

Entry
Database: PDB / ID: 2wnm
TitleSolution structure of Gp2
ComponentsGENE 2
KeywordsHYDROLASE / SMALL PROTEIN INHIBNTOR BACTERIAL RNA POLYMERASE
Function / homology
Function and homology information


symbiont-mediated suppression of host transcription
Similarity search - Function
RNA polymerase inhibitor / RNA polymerase inhibitor / RNA polymerase inhibitor superfamily / RNA polymerase inhibitor / Ubiquitin-like (UB roll) / Roll / Alpha Beta
Similarity search - Domain/homology
Bacterial RNA polymerase inhibitor / Gene 2
Similarity search - Component
Biological speciesENTEROBACTERIA PHAGE T7 (virus)
MethodSOLUTION NMR / ARIA
Model type detailsMINIMIZED AVERAGE
AuthorsCamara, B. / Liu, M. / Shadrinc, A. / Liu, B. / Simpson, P. / Weinzierl, R. / Severinovc, K. / Cota, E. / Matthews, S. / Wigneshweraraj, S.R.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2010
Title: T7 Phage Protein Gp2 Inhibits the Escherichia Coli RNA Polymerase by Antagonizing Stable DNA Strand Separation Near the Transcription Start Site.
Authors: Camara, B. / Liu, M. / Reynolds, J. / Shadrin, A. / Liu, B. / Kwok, K. / Simpson, P. / Weinzierl, R. / Severinov, K. / Cota, E. / Matthews, S. / Wigneshweraraj, S.R.
History
DepositionJul 13, 2009Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 16, 2010Provider: repository / Type: Initial release
Revision 1.1May 8, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: GENE 2


Theoretical massNumber of molelcules
Total (without water)7,1811
Polymers7,1811
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / 20REPRESENTATIVE STRUCTURE
RepresentativeModel #1

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Components

#1: Protein GENE 2 / / GP2


Mass: 7180.985 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) ENTEROBACTERIA PHAGE T7 (virus) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q6WYQ8, UniProt: P03704*PLUS

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: NONE

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Sample preparation

Sample conditionsTemperature: 310.0 K

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NMR measurement

NMR spectrometerType: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 800 MHz

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Processing

NMR software
NameVersionDeveloperClassification
CNS1.1BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- KUNSTLEVE,JIANG,KUSZEWSKI,NILGES,PANNU, READ,RICE,SIMONSON,WARRENrefinement
CNSstructure solution
RefinementMethod: ARIA / Software ordinal: 1
NMR ensembleConformer selection criteria: REPRESENTATIVE STRUCTURE / Conformers calculated total number: 20 / Conformers submitted total number: 1

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