+Open data
-Basic information
Entry | Database: PDB / ID: 2n2q | ||||||
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Title | NMR solution structure of HsAFP1 | ||||||
Components | Defensin-like protein 1 | ||||||
Keywords | ANTIFUNGAL PROTEIN | ||||||
Function / homology | Function and homology information defense response to fungus / killing of cells of another organism / extracellular region Similarity search - Function | ||||||
Biological species | Heuchera sanguinea (coralbells) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Harvey, P.J. / Craik, D.J. / Vriens, K. | ||||||
Citation | Journal: Plos One / Year: 2015 Title: Synergistic Activity of the Plant Defensin HsAFP1 and Caspofungin against Candida albicans Biofilms and Planktonic Cultures. Authors: Vriens, K. / Cools, T.L. / Harvey, P.J. / Craik, D.J. / Spincemaille, P. / Cassiman, D. / Braem, A. / Vleugels, J. / Nibbering, P.H. / Drijfhout, J.W. / De Coninck, B. / Cammue, B.P. / Thevissen, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n2q.cif.gz | 302.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n2q.ent.gz | 263 KB | Display | PDB format |
PDBx/mmJSON format | 2n2q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n2/2n2q ftp://data.pdbj.org/pub/pdb/validation_reports/n2/2n2q | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5958.750 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Heuchera sanguinea (coralbells) / Production host: Pichia pastoris (fungus) / References: UniProt: P0C8Y5 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 4 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker Avance / Manufacturer: Bruker / Model: Avance / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |