- EMDB-10051: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaet... -
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基本情報
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データベース: EMDB / ID: EMD-10051
タイトル
Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state A
マップデータ
試料
90S pre-ribosome, state A
Periodic tryptophan protein 2-like protein
Utp2
Utp3
Utp4
Utp6
Utp7
UTP10
U3 small nucleolar RNA-associated protein 11
Utp12
Utp13"
Utp14
Utp15
Utp17
Utp18
Putative U3 snoRNP protein
Utp24
Utp30
Nop1
Putative nucleolar protein核小体
Nop58
snu13NHP2L1
Rrp9
RNA 3'-terminal phosphate cyclase-like protein
Bms1
Imp3
(Putative U3 small nucleolar ...) x 2
Sof1
Emg1
KRR1 small subunit processome component
Pre-rRNA-processing protein PNO1
Kre33
Fcf2
(40S ribosomal protein ...) x 10
Faf1
(nucleic-acid核酸) x 2
Utp8
Utp5"
Noc4
Putative ribosomal protein
Enp1
Utp16
(ligandリガンド) x 3
機能・相同性
機能・相同性情報
rRNA cytidine N-acetyltransferase activity / tRNA acetylation / mRNA modification / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex ...rRNA cytidine N-acetyltransferase activity / tRNA acetylation / mRNA modification / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex / snRNA binding / rRNA base methylation / protein localization to nucleolus / U3 snoRNA binding / rRNA export from nucleus / sno(s)RNA-containing ribonucleoprotein complex / 90S preribosome / preribosome, small subunit precursor / snoRNA binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 転写後修飾 / small-subunit processome / ribosomal small subunit biogenesis / nuclear periphery / 転移酵素; アシル基を移すもの; アミノアシル基以外のアシル基を移すもの / 助触媒 / small ribosomal subunit rRNA binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rRNA processing / 転写後修飾 / リボソーム生合成 / methyltransferase activity / cytosolic small ribosomal subunit / ribonucleoprotein complex / small ribosomal subunit / tRNA binding / メチル化 / rRNA binding / リボソーム / structural constituent of ribosome / protein ubiquitination / 翻訳 (生物学) / GTPase activity / GTP binding / 核小体 / RNA binding / 生体膜 / ATP binding / identical protein binding / 細胞核 / 細胞質
Fcf2 pre-rRNA processing, C-terminal / BING4, C-terminal domain / Quinoprotein alcohol dehydrogenase-like superfamily / Tetratricopeptide-like helical domain superfamily / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / Nucleic acid-binding, OB-fold / Ribosomal protein S13/S15, N-terminal / AARP2CN / BP28, C-terminal domain / Ribosomal protein S13-like, H2TH ...Fcf2 pre-rRNA processing, C-terminal / BING4, C-terminal domain / Quinoprotein alcohol dehydrogenase-like superfamily / Tetratricopeptide-like helical domain superfamily / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / Nucleic acid-binding, OB-fold / Ribosomal protein S13/S15, N-terminal / AARP2CN / BP28, C-terminal domain / Ribosomal protein S13-like, H2TH / NOP5, N-terminal / NOSIC / U3 snoRNA associated / tRNA(Met) cytidine acetyltransferase TmcA, N-terminal / RNA 3'-terminal phosphate cyclase, insert domain / RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta / Quinoprotein amine dehydrogenase, beta chain-like / S15/NS1, RNA-binding / U3 small nucleolar RNA-associated protein 6 / Brix domain / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S12/S23 / Small-subunit processome, Utp14 / Ribosome biogenesis protein BMS1/TSR1, C-terminal / Small-subunit processome, Utp11 / Bystin / Sas10/Utp3/C1D / Small-subunit processome, Utp12 / Nucleolar protein 14 / Sof1-like protein / Small-subunit processome, Utp21 / Helicase domain / Small-subunit processome, Utp13 / Ribosomal protein S5 domain 2-type fold, subgroup / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / Ribosomal protein S7 domain / Fibrillarin, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosomal protein S4/S9 / Ribosomal protein S15P / Ribosomal protein L1-like / RNA 3'-terminal phosphate cyclase domain / Ribosomal RNA assembly KRR1 / Ribosomal protein S9, conserved site / Periodic tryptophan protein 2 / Nucleolar complex protein 4 / P-loop containing nucleoside triphosphate hydrolase / 30s ribosomal protein S13, C-terminal / 40S ribosomal protein S1/3, eukaryotes / Possible tRNA binding domain / Ribosomal protein S7, conserved site / Ribosomal protein S5 domain 2-type fold / Helix hairpin bin domain superfamily / Ribosomal S11, conserved site / WD40/YVTN repeat-like-containing domain superfamily / Armadillo-type fold / Ribosomal protein L1, 3-layer alpha/beta-sandwich / RNA 3'-terminal phosphate cyclase type 2 / WD40-repeat-containing domain / Ribosomal protein S4, conserved site / Ribosomal protein S13, conserved site / G-protein beta WD-40 repeat / Ribosomal protein S3Ae, conserved site / Pyrrolo-quinoline quinone beta-propeller repeat / Ribosomal protein L7Ae/L8/Nhp2 family / Sas10 C-terminal domain / U3 small nucleolar RNA-associated protein 15, C-terminal / WD40 repeat, conserved site / CCAAT-binding factor / K Homology domain / Ribosomal protein S28e conserved site / Ribosomal protein S11 superfamily / Bms1/Tsr1-type G domain / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / TmcA/NAT10/Kre33 / RNA cytidine acetyltransferase NAT10 / Ribosomal protein S8 superfamily / Nop domain superfamily / WD40-repeat-containing domain superfamily / RNA 3'-terminal phosphate cyclase, insert domain superfamily / K Homology domain, type 1 superfamily / Ribosomal protein S7 domain superfamily / RNA-binding S4 domain superfamily / S-adenosyl-L-methionine-dependent methyltransferase / RNA 3'-terminal phosphate cyclase domain superfamily / Ribosome biogenesis protein Bms1, N-terminal / Ribosomal RNA-processing protein Rrp9-like / Ribosome biogenesis protein Bms1/Tsr1 / Fcf2/DNTTIP2 / Pre-rRNA-processing protein PNO1-like / U3 small nucleolar RNA-associated protein 10 / WD repeat-containing protein WDR46/Utp7 / Krr1, KH1 domain / Nop, C-terminal domain / 50S ribosomal protein L30e-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein S15 / Fibrillarin
40S ribosomal protein S7 / Uncharacterized protein / Putative U3 small nucleolar ribonucleoprotein protein / 40S ribosomal protein S28-like protein / Uncharacterized protein / WD_REPEATS_REGION domain-containing protein / 40S ribosomal protein S16-like protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / S4 RNA-binding domain-containing protein ...40S ribosomal protein S7 / Uncharacterized protein / Putative U3 small nucleolar ribonucleoprotein protein / 40S ribosomal protein S28-like protein / Uncharacterized protein / WD_REPEATS_REGION domain-containing protein / 40S ribosomal protein S16-like protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / S4 RNA-binding domain-containing protein / Putative U3 small nucleolar ribonucleoprotein / Uncharacterized protein / WD_REPEATS_REGION domain-containing protein / U3 small nucleolar RNA-associated protein 11 / Periodic tryptophan protein 2-like protein / 40S ribosomal protein S14-like protein / Uncharacterized protein / WD_REPEATS_REGION domain-containing protein / Ribonucloprotein / Histone-glutamine methyltransferase / 40S ribosomal protein S22-like protein / Uncharacterized protein / Putative U3 snoRNP protein / RNA cytidine acetyltransferase / 40S ribosomal protein S1 / 40S ribosomal protein s5-like protein / 40S ribosomal protein s23-like protein / DDB1- and CUL4-associated factor 13 / Nucleolar protein 58 / Utp12 domain-containing protein / WD_REPEATS_REGION domain-containing protein / CBF domain-containing protein / WD_REPEATS_REGION domain-containing protein / 40S ribosomal protein S13-like protein / RNA 3'-terminal phosphate cyclase-like protein / 40S ribosomal protein s9-like protein / Nucleolar protein 58 / Putative ribosomal protein / Uncharacterized protein / Pre-rRNA-processing protein PNO1 / KRR1 small subunit processome component / Bms1-type G domain-containing protein / Fcf2 domain-containing protein / Sas10 domain-containing protein / Uncharacterized protein / Uncharacterized protein / U3 small nucleolar RNA-associated protein 10 / Uncharacterized protein / Uncharacterized protein CTHT_0071780
ジャーナル: Mol Cell / 年: 2019 タイトル: Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration. 著者: Jingdong Cheng / Jochen Baßler / Paulina Fischer / Benjamin Lau / Nikola Kellner / Ruth Kunze / Sabine Griesel / Martina Kallas / Otto Berninghausen / Daniela Strauss / Roland Beckmann / Ed Hurt / 要旨: Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome ...Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.