+Open data
-Basic information
Entry | Database: PDB / ID: 1sto | ||||||
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Title | CRYSTAL STRUCTURE OF OROTATE PHOSPHORIBOSYLTRANSFERASE | ||||||
Components | OROTATE PHOSPHORIBOSYLTRANSFERASE | ||||||
Keywords | PHOSPHORIBOSYLTRANSFERASE | ||||||
Function / homology | Function and homology information pyrimidine ribonucleoside biosynthetic process / orotate phosphoribosyltransferase / orotate phosphoribosyltransferase activity / pyrimidine nucleotide biosynthetic process / 'de novo' UMP biosynthetic process / 'de novo' pyrimidine nucleobase biosynthetic process / magnesium ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Salmonella typhimurium (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.6 Å | ||||||
Authors | Scapin, G. / Grubmeyer, C. / Sacchettini, J.C. | ||||||
Citation | Journal: Biochemistry / Year: 1994 Title: Crystal structure of orotate phosphoribosyltransferase. Authors: Scapin, G. / Grubmeyer, C. / Sacchettini, J.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sto.cif.gz | 50.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sto.ent.gz | 39.6 KB | Display | PDB format |
PDBx/mmJSON format | 1sto.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/st/1sto ftp://data.pdbj.org/pub/pdb/validation_reports/st/1sto | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 23533.936 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhimurium (bacteria) References: UniProt: P08870, orotate phosphoribosyltransferase |
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#2: Chemical | ChemComp-OH / |
#3: Chemical | ChemComp-OMP / |
#4: Water | ChemComp-HOH / |
Sequence details | SEQUENCE ADVISORY NOTICE DIFFERENCE BETWEEN SWISS-PROT AND PDB SEQUENCE. SWISS-PROT ENTRY NAME: ...SEQUENCE ADVISORY NOTICE DIFFERENCE |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 52.02 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.5 Å / Num. obs: 6839 / % possible obs: 60 % / Rmerge(I) obs: 0.062 |
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-Processing
Software |
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Refinement | Resolution: 2.6→28 Å /
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Refinement step | Cycle: LAST / Resolution: 2.6→28 Å
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Refine LS restraints |
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Refinement | *PLUS σ(F): 1 / Rfactor obs: 0.176 / Rfactor Rwork: 0.176 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2.5 |