SASDAL4: CRM1 RanGTP Snu1 (Exportin-1 + GTP-binding nuclear protein Ran + ...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAL4

試料

CRM1 RanGTP Snu1

• Exportin-1 (protein), Mus musculus
• GTP-binding nuclear protein Ran (protein), Homo sapiens
• Snurportin-1 (protein), Homo sapiens

機能・相同性

分子機能:

Estrogen-dependent nuclear events downstream of ESR-membrane signaling / Cyclin A/B1/B2 associated events during G2/M transition / HuR (ELAVL1) binds and stabilizes mRNA / Heme signaling / RNA import into nucleus / Deactivation of the beta-catenin transactivating complex / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / Transcriptional and post-translational regulation of MITF-M expression and activity / Mitotic Prometaphase / EML4 and NUDC in mitotic spindle formation / Resolution of Sister Chromatid Cohesion / annulate lamellae / RHO GTPases Activate Formins / regulation of proteasomal ubiquitin-dependent protein catabolic process / Separation of Sister Chromatids / MAPK6/MAPK4 signaling / RNA cap binding / pre-miRNA export from nucleus / RNA nuclear export complex / snRNA import into nucleus / regulation of centrosome duplication / nuclear export signal receptor activity / Regulation of cholesterol biosynthesis by SREBP (SREBF) / NLS-dependent protein nuclear import complex / regulation of protein export from nucleus / protein localization to nucleolus / Rev-mediated nuclear export of HIV RNA / Nuclear import of Rev protein / NEP/NS2 Interacts with the Cellular Export Machinery / GTP metabolic process / tRNA processing in the nucleus / Postmitotic nuclear pore complex (NPC) reformation / nuclear import signal receptor activity / MicroRNA (miRNA) biogenesis / DNA metabolic process / regulation of protein catabolic process / protein complex oligomerization / mitotic sister chromatid segregation / ribosomal large subunit export from nucleus / protein localization to nucleus / viral process / nuclear pore / ribosomal subunit export from nucleus / mRNA export from nucleus / Cajal body / ribosomal small subunit export from nucleus / cytoskeleton organization / centriole / protein export from nucleus / mitotic spindle organization / protein tetramerization / Transcriptional regulation by small RNAs / recycling endosome / small GTPase binding / kinetochore / positive regulation of protein import into nucleus / protein import into nucleus / GDP binding / positive regulation of protein binding / nuclear envelope / melanosome / mitotic cell cycle / G protein activity / snRNP Assembly / midbody / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / nuclear membrane / cadherin binding / protein heterodimerization activity / ribonucleoprotein complex / cell division / GTPase activity / chromatin binding / GTP binding / chromatin / nucleolus / magnesium ion binding / protein-containing complex / RNA binding / extracellular exosome / nucleoplasm / nucleus / membrane / plasma membrane / cytosol / cytoplasm

ドメイン・相同性:

Snurportin-1 / Snurportin-1, N-terminal / : / Snurportin1 / Snurportin1, m3G cap-binding domain / Exportin-1, repeat 3 / Chromosome region maintenance repeat / Exportin-1, repeat 2 / Chromosome region maintenance or exportin repeat / CRM1 / Exportin repeat 2 / CRM1 / Exportin repeat 3 / CRM1 C terminal / Exportin-1, C-terminal / CRM1 C terminal / Exportin-1/5 / Exportin-1/Importin-beta-like / Exportin 1-like protein / small GTPase Ran family profile. / Ran GTPase / Importin-beta N-terminal domain / Importin-beta N-terminal domain / Importin-beta N-terminal domain profile. / Importin-beta, N-terminal domain / Importin-alpha, importin-beta-binding domain / IBB domain profile. / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / Armadillo-like helical / Small GTP-binding protein domain / Armadillo-type fold / P-loop containing nucleoside triphosphate hydrolase

構成要素:

Snurportin-1 / GTP-binding nuclear protein Ran / Exportin-1

• 生物種: Mus musculus (ハツカネズミ); Homo sapiens (ヒト)
• 引用: ジャーナル: Structure / 年: 2013; タイトル: Structural determinants and mechanism of mammalian CRM1 allostery.; 著者: Nicole Dölker / Clement E Blanchet / Béla Voß / David Haselbach / Christian Kappel / Thomas Monecke / Dmitri I Svergun / Holger Stark / Ralf Ficner / Ulrich Zachariae / Helmut Grubmüller / Achim Dickmanns / ; 要旨: Proteins carrying nuclear export signals cooperatively assemble with the export factor CRM1 and the effector protein RanGTP. In lower eukaryotes, this cooperativity is coupled to CRM1 conformational changes; however, it is unknown if mammalian CRM1 maintains its compact conformation or shows similar structural flexibility. Here, combinations of small-angle X-ray solution scattering and electron microscopy experiments with molecular dynamics simulations reveal pronounced conformational flexibility in mammalian CRM1 and demonstrate that RanGTP binding induces association of its N- and C-terminal regions to form a toroid structure. The CRM1 toroid is stabilized mainly by local interactions between the terminal regions, rather than by global strain. The CRM1 acidic loop is key in transmitting the effect of this RanGTP-induced global conformational change to the NES-binding cleft by shifting its population to the open state, which displays enhanced cargo affinity. Cooperative CRM1 export complex assembly thus constitutes a highly dynamic process, encompassing an intricate interplay of global and local structural changes.

登録者

• Clement Blanchet (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #70: ; タイプ: dummy / ソフトウェア: Dammif / ダミー原子の半径: 1.90 A; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #74: ; タイプ: dummy / ソフトウェア: Monsa / ダミー原子の半径: 1.90 A / カイ2乗値: 1.5625; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #75: ; タイプ: dummy / ソフトウェア: Monsa / ダミー原子の半径: 1.90 A / カイ2乗値: 1.550025; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: CRM1 RanGTP Snu1 / Sample MW: 188.65 kDa / 試料濃度: 1.00-7.00 / Entity id: 62 / 63 / 64
• バッファ: 名称: 50 mM Tris-HCL / pH: 7.5 / 組成: 150 mM NaCl, 1.0 mM DTT

要素 #62

タイプ: protein / 記述: Exportin-1 / 分子量: 123.09 / 分子数: 1 / 由来: Mus musculus / 参照: UniProt: Q6P5F9
配列:

MPAIMTMLAD HAARQLLDFS QKLDINLLDN VVNCLYHGEG AQQRMAQEVL THLKEHPDAW TRVDTILEFS QNMNTKYYGL QILENVIKTR WKILPRNQCE GIKKYVVGLI IKTSSDPTCV EKEKVYIGKL NMILVQILKQ EWPKHWPTFI SDIVGASRTS ESLCQNNMVI LKLLSEEVFD FSSGQITQVK AKHLKDSMCN EFSQIFQLCQ FVMENSQNAP LVHATLETLL RFLNWIPLGY IFETKLISTL IYKFLNVPMF RNVSLKCLTE IAGVSVSQYE EQFETLFTLT MMQLKQMLPL NTNIRLAYSN GKDDEQNFIQ NLSLFLCTFL KEHGQLLEKR LNLREALMEA LHYMLLVSEV EETEIFKICL EYWNHLAAEL YRESPFSTSA SPLLSGSQHF DIPPRRQLYL TVLSKVRLLM VSRMAKPEEV LVVENDQGEV VREFMKDTDS INLYKNMRET LVYLTHLDYV DTEIIMTKKL QNQVNGTEWS WKNLNTLCWA IGSISGAMHE EDEKRFLVTV IKDLLGLCEQ KRGKDNKAII ASNIMYIVGQ YPRFLRAHWK FLKTVVNKLF EFMHETHDGV QDMACDTFIK IAQKCRRHFV QVQVGEVMPF IDEILNNINT IICDLQPQQV HTFYEAVGYM IGAQTDQTVQ EHLIEKYMLL PNQVWDSIIQ QATKNVDILK DPETVKQLGS ILKTNVRACK AVGHPFVIQL GRIYLDMLNV YKCLSENISA AIQANGEMVT KQPLIRSMRT VKRETLKLIS GWVSRSNDPQ MVAENFVPPL LDAVLIDYQR NVPAAREPEV LSTMAIIVNK LGGHITAEIP QIFDAVFECT LNMINKDFEE YPEHRTNFFL LLQAVNSHCF PAFLAIPPAQ FKLVLDSIIW AFKHTMRNVA DTGLQILFTL LQNVAQEEAA AQSFYQTYFC DILQHIFSVV TDTSHTAGLT MHASILAYMF NLVEEGKIST PLNPGNPVNN QMFIQDYVAN LLKSAFPHLQ DAQVKLFVTG LFSLNQDIPA FKEHLRDFLV QIKEFAGEDT SDLFLEERET ALRQAQEEKH KLQMSVPGIL NPHEIPEEMC D

要素 #63

タイプ: protein / 記述: GTP-binding nuclear protein Ran / 分子量: 24.42 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P62826
配列:

MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL

要素 #64

タイプ: protein / 記述: Snurportin-1 / 分子量: 41.14 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: O95149
配列:

MEELSQALAS SFSVSQDLNS TAAPHPRLSQ YKSKYSSLEQ SERRRRLLEL QKSKRLDYVN HARRLAEDDW TGMESEEENK KDDEEMDIDT VKKLPKHYAN QLMLSEWLID VPSDLGQEWI VVVCPVGKRA LIVASRGSTS AYTKSGYCVN RFSSLLPGGN RRNSTAKDYT ILDCIYNEVN QTYYVLDVMC WRGHPFYDCQ TDFRFYWMHS KLPEEEGLGE KTKLNPFKFV GLKNFPCTPE SLCDVLSMDF PFEVDGLLFY HKQTHYSPGS TPLVGWLRPY MVSDVLGVAV PAGPLTTKPD YAGHQLQQIM EHKKSQKEGM KEKLTHKASE NGHYELEHLS TPKLKGSSHS PDHPGCLMEN

実験情報

• ビーム: 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron
• 検出器: 名称: MAR 345 Image Plate

スキャン

タイトル: CRM1 + RanGTP + SPN1 / 測定日: 2009年2月3日 / セル温度: 10 °C / 照射時間: 120 sec. / フレーム数: 1 / 単位: 1/nm /

	Min	Max
Q	0.1168	4.943

結果

カーブのタイプ: single_conc /

	実験値	Porod
分子量	190 kDa	190 kDa

	Guinier	P(R)
前方散乱 I0	1.771	-
慣性半径, Rg	4.141 nm	4.1 nm

	Min	Max
D	-	14
Guinier point	29	87