+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDAC6 |
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試料 | PsrP functional binding region
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機能・相同性 | 機能・相同性情報 Gram-positive-bacterium-type cell wall / symbiont-mediated perturbation of host defense response / single-species biofilm formation / cell adhesion / cell surface / DNA binding / extracellular region 類似検索 - 分子機能 |
生物種 | Streptococcus pneumoniae (肺炎レンサ球菌) |
引用 | ジャーナル: Sci Rep / 年: 2016 タイトル: The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. 著者: Tim Schulte / Cecilia Mikaelsson / Audrey Beaussart / Alexey Kikhney / Maya Deshmukh / Sebastian Wolniak / Anuj Pathak / Christine Ebel / Jonas Löfling / Federico Fogolari / Birgitta ...著者: Tim Schulte / Cecilia Mikaelsson / Audrey Beaussart / Alexey Kikhney / Maya Deshmukh / Sebastian Wolniak / Anuj Pathak / Christine Ebel / Jonas Löfling / Federico Fogolari / Birgitta Henriques-Normark / Yves F Dufrêne / Dmitri Svergun / Per-Åke Nygren / Adnane Achour / 要旨: The major human pathogen Streptococcus pneumoniae is a leading cause of disease and death worldwide. Pneumococcal biofilm formation within the nasopharynx leads to long-term colonization and ...The major human pathogen Streptococcus pneumoniae is a leading cause of disease and death worldwide. Pneumococcal biofilm formation within the nasopharynx leads to long-term colonization and persistence within the host. We have previously demonstrated that the capsular surface-associated pneumococcal serine rich repeat protein (PsrP), key factor for biofilm formation, binds to keratin-10 (KRT10) through its microbial surface component recognizing adhesive matrix molecule (MSCRAMM)-related globular binding region domain (BR187-385). Here, we show that BR187-385 also binds to DNA, as demonstrated by electrophoretic mobility shift assays and size exclusion chromatography. Further, heterologous expression of BR187-378 or the longer BR120-378 construct on the surface of a Gram-positive model host bacterium resulted in the formation of cellular aggregates that was significantly enhanced in the presence of DNA. Crystal structure analyses revealed the formation of BR187-385 homo-dimers via an intermolecular β-sheet, resulting in a positively charged concave surface, shaped to accommodate the acidic helical DNA structure. Furthermore, small angle X-ray scattering and circular dichroism studies indicate that the aggregate-enhancing N-terminal region of BR120-166 adopts an extended, non-globular structure. Altogether, our results suggest that PsrP adheres to extracellular DNA in the biofilm matrix and thus promotes pneumococcal biofilm formation. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #565 | タイプ: mix / ソフトウェア: EOM (1.3) / ダミー原子の半径: 1.90 A / カイ2乗値: 0.974169 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #566 | タイプ: mix / ソフトウェア: EOM (1.4) / ダミー原子の半径: 1.90 A / カイ2乗値: 0.974169 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: PsrP functional binding region / 試料濃度: 2.9 mg/ml |
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バッファ | 名称: PBS / pH: 7.4 / コメント: Phosphate buffered saline / 組成: Glycerol 5.000 % |
要素 #37 | 名称: PsrP BR(187-385) / タイプ: protein 記述: Functional binding region (187-385) of the pneumococcal serine-rich repeat protein 分子量: 22.12 / 分子数: 1 / 由来: Streptococcus pneumoniae / 参照: UniProt: A0A0H2URK1 配列: HHHHHHSGNT IVNGAPAINA SLNIAKSETK VYTGEGVDSV YRVPIYYKLK VTNDGSKLTF TYTVTYVNPK TNDLGNISSM RPGYSIYNSG TSTQTMLTLG SDLGKPSGVK NYITDKNGRQ VLSYNTSTMT TQGSGYTWGN GAQMNGFFAK KGYGLTSSWT VPITGTDTSF ...配列: HHHHHHSGNT IVNGAPAINA SLNIAKSETK VYTGEGVDSV YRVPIYYKLK VTNDGSKLTF TYTVTYVNPK TNDLGNISSM RPGYSIYNSG TSTQTMLTLG SDLGKPSGVK NYITDKNGRQ VLSYNTSTMT TQGSGYTWGN GAQMNGFFAK KGYGLTSSWT VPITGTDTSF TFTPYAARTD RIGINYFNGG GKVVESSTTS QSLSQ |
-実験情報
ビーム | 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.09919 Å / スペクトロメータ・検出器間距離: 2.43 mm | ||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M | ||||||||||||||||||||||||||||||
スキャン | タイトル: PsrP functional binding region / 測定日: 2013年2月27日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.6 / ポイント数: 429 /
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結果 | カーブのタイプ: single_conc / Standard: BSA
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