+
データを開く
-
基本情報
登録情報 | データベース: SASBDB / ID: SASDAB9 |
---|---|
![]() | EcPaaA2-HisEcParE2 construct
|
機能・相同性 | ParE toxin of type II toxin-antitoxin system, parDE / Toxin-antitoxin system, RelE/ParE toxin family / Toxin-antitoxin system, RelE/ParE toxin domain superfamily / Plasmid stabilization protein ParE / : ![]() |
生物種 | ![]() ![]() |
![]() | ![]() タイトル: A unique hetero-hexadecameric architecture displayed by the Escherichia coli O157 PaaA2-ParE2 antitoxin-toxin complex. 著者: Yann G-J Sterckx / Thomas Jové / Alexander V Shkumatov / Abel Garcia-Pino / Lieselotte Geerts / Maia De Kerpel / Jurij Lah / Henri De Greve / Laurence Van Melderen / Remy Loris / ![]() ![]() 要旨: Many bacterial pathogens modulate their metabolic activity, virulence and pathogenicity through so-called "toxin-antitoxin" (TA) modules. The genome of the human pathogen Escherichia coli O157 ...Many bacterial pathogens modulate their metabolic activity, virulence and pathogenicity through so-called "toxin-antitoxin" (TA) modules. The genome of the human pathogen Escherichia coli O157 contains two three-component TA modules related to the known parDE module. Here, we show that the toxin EcParE2 maps in a branch of the RelE/ParE toxin superfamily that is distinct from the branches that contain verified gyrase and ribosome inhibitors. The structure of EcParE2 closely resembles that of Caulobacter crescentus ParE but shows a distinct pattern of conserved surface residues, in agreement with its apparent inability to interact with GyrA. The antitoxin EcPaaA2 is characterized by two α-helices (H1 and H2) that serve as molecular recognition elements to wrap itself around EcParE2. Both EcPaaA2 H1 and H2 are required to sustain a high-affinity interaction with EcParE2 and for the inhibition of EcParE2-mediated killing in vivo. Furthermore, evidence demonstrates that EcPaaA2 H2, but not H1, determines specificity for EcParE2. The initially formed EcPaaA2-EcParE2 heterodimer then assembles into a hetero-hexadecamer, which is stable in solution and is formed in a highly cooperative manner. Together these findings provide novel data on quaternary structure, TA interactions and activity of a hitherto poorly characterized family of TA modules. |
![]() |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-モデル
モデル #355 | ![]() タイプ: mix / ダミー原子の半径: 1.90 A / コメント: NMA refinement / カイ2乗値: 3.439 ![]() |
---|---|
モデル #356 | ![]() タイプ: dummy / ダミー原子の半径: 3.50 A / カイ2乗値: 0.637 ![]() |
-
試料
![]() | 名称: EcPaaA2-HisEcParE2 construct / 試料濃度: 1.00-10.00 / Entity id: 215 / 216 |
---|---|
バッファ | 名称: 50 mM Tris-HCl 500 mM NaCl / 濃度: 50.00 mM / pH: 7.5 / 組成: 500 mM NaCl |
要素 #215 | 名称: EcParE2 / タイプ: protein / 記述: Plasmid stabilization protein ParE / 分子量: 12.693 / 分子数: 8 / 由来: Escherichia coli / 参照: UniProt: A0A0D7C2L1 配列: MGSSHHHHHH SSGLVPRGSH LPVLWLESAD TDLDDITSYI ARFDIDAAER LWQRLRGCVL PLSEHPYLYP PSDRVPGLRE IVAHPNYIIL YRVTTSSVEV VNVIHARRQF P |
要素 #216 | 名称: EcPaaA2 / タイプ: protein / 記述: Uncharacterized protein (Antitoxin) / 分子量: 8.458 / 分子数: 8 / 由来: Escherichia coli / 参照: UniProt: A0A0F6F6Q9 配列: MDYKDDDDKN RALSPMVSEF ETIEQENSYN EWLRAKVATS LADPRPAIPH DEVERRMAER FAKMRKERSK Q |
-実験情報
ビーム | 設備名称: SOLEIL SWING ![]() ![]() | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: AVIEX / タイプ: CCD | |||||||||||||||||||||
スキャン |
| |||||||||||||||||||||
距離分布関数 P(R) |
| |||||||||||||||||||||
結果 | コメント: Use of SAXS to validate the oligomer state of the obtained crystal structure and to model missing afinity tags
|