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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDFL8 |
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![]() | GON7, the fifth subunit of human KEOPS, bound to LAGE3-OSGEP (EKC/KEOPS complex subunit:Probable tRNA N6-adenosine threonylcarbamoyltransferase)
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機能・相同性 | ![]() N6-L-threonylcarbamoyladenine synthase / N(6)-L-threonylcarbamoyladenine synthase activity / EKC/KEOPS complex / tRNA threonylcarbamoyladenosine modification / tRNA modification in the nucleus and cytosol / nuclear speck / nucleolus / nucleoplasm / nucleus / metal ion binding ...N6-L-threonylcarbamoyladenine synthase / N(6)-L-threonylcarbamoyladenine synthase activity / EKC/KEOPS complex / tRNA threonylcarbamoyladenosine modification / tRNA modification in the nucleus and cytosol / nuclear speck / nucleolus / nucleoplasm / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能 |
生物種 | ![]() |
![]() | ![]() タイトル: Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome 著者: Arrondel C / Missoury S / Snoek R / Patat J / Menara G / Collinet B / Liger D / Durand D / Gribouval O / Boyer O / Buscara L / Martin G / Machuca E / Nevo F / Lescop E / Braun D / Boschat A / ...著者: Arrondel C / Missoury S / Snoek R / Patat J / Menara G / Collinet B / Liger D / Durand D / Gribouval O / Boyer O / Buscara L / Martin G / Machuca E / Nevo F / Lescop E / Braun D / Boschat A / Sanquer S / Guerrera I / Revy P / Parisot M / Masson C / Boddaert N / Charbit M / Decramer S / Novo R / Macher M / Ranchin B / Bacchetta J / Laurent A / Collardeau-Frachon S / van Eerde A / Hildebrandt F / Magen D / Antignac C / van Tilbeurgh H |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #3207 | ![]() タイプ: atomic / カイ2乗値: 0.308 / P-value: 0.001285 ![]() |
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試料
![]() | 名称: GON7, the fifth subunit of human KEOPS, bound to LAGE3-OSGEP (EKC/KEOPS complex subunit:Probable tRNA N6-adenosine threonylcarbamoyltransferase) 試料濃度: 1.48 mg/ml / Entity id: 1759 / 1760 / 1761 |
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バッファ | 名称: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol / pH: 6.5 |
要素 #1759 | 名称: GON7 / タイプ: protein / 記述: EKC/KEOPS complex subunit GON7 / 分子量: 11.682 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: Q9BXV9 配列: MELLGEYVGQ EGKPQKLRVS CEAPGDGDPF QGLLSGVAQM KDMVTELFDP LVQGEVQHRV AAAPDEDLDG DDEDDAEDEN NIDNRTNFDG PSAKRPKTPS HHHHHH |
要素 #1760 | 名称: LAGE3 / タイプ: protein / 記述: EKC/KEOPS complex subunit LAGE3 / 分子量: 14.804 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: 1-143 配列: MRDADADAGG GADGGDGRGG HSCRGGVDTA AAPAGGAPPA HAPGPGRDAA SAARGSRMRP HIFTLSVPFP TPLEAEIAHG SLAPDAEPHQ RVVGKDLTVS GRILVVRWKA EDCRLLRISV INFLDQLSLV VRTMQRFGPP VSR |
要素 #1761 | 名称: OSGEP / タイプ: protein 記述: Probable tRNA N6-adenosine threonylcarbamoyltransferase 分子量: 36.426 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: Q9NPF4 配列: MPAVLGFEGS ANKIGVGVVR DGKVLANPRR TYVTPPGTGF LPGDTARHHR AVILDLLQEA LTESGLTSQD IDCIAYTKGP GMGAPLVSVA VVARTVAQLW NKPLVGVNHC IGHIEMGRLI TGATSPTVLY VSGGNTQVIA YSEHRYRIFG ETIDIAVGNC LDRFARVLKI ...配列: MPAVLGFEGS ANKIGVGVVR DGKVLANPRR TYVTPPGTGF LPGDTARHHR AVILDLLQEA LTESGLTSQD IDCIAYTKGP GMGAPLVSVA VVARTVAQLW NKPLVGVNHC IGHIEMGRLI TGATSPTVLY VSGGNTQVIA YSEHRYRIFG ETIDIAVGNC LDRFARVLKI SNDPSPGYNI EQMAKRGKKL VELPYTVKGM DVSFSGILSF IEDVAHRMLA TGECTPEDLC FSLQETVFAM LVEITERAMA HCGSQEALIV GGVGCNVRLQ EMMATMCQER GARLFATDER FCIDNGAMIA QAGWEMFRAG HRTPLSDSGV TQRYRTDEVE VTWRD |
-実験情報
ビーム | 設備名称: SOLEIL SWING ![]() ![]() | |||||||||||||||||||||||||||||||||
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検出器 | 名称: AVIEX PCCD170170 / タイプ: CCD | |||||||||||||||||||||||||||||||||
スキャン | 測定日: 2017年5月12日 / セル温度: 15 °C / 照射時間: 1 sec. / フレーム数: 255 / 単位: 1/A /
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距離分布関数 P(R) |
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結果 | コメント: The scattered intensities were displayed on an absolute scale (cm-1) using the scattering of water. SAXS data were normalized to the intensity of the incident beam, averaged and ...コメント: The scattered intensities were displayed on an absolute scale (cm-1) using the scattering of water. SAXS data were normalized to the intensity of the incident beam, averaged and background subtracted using the program US-SOMO. Frames were examined individually and 21 identical frames were averaged and further processed. The corresponding SEC-elution concentration was approximately 0.35 mg/mL. The complex is constituted of one monomer of OSGEP, one monomer of LAGE3 and one monomer of GON7. Molecular mass was obtained using the Bayesian inference approach proposed in PRIMUS/qt, which combines four concentration independent MM estimators: M=59.5 kDa within the credibility interval [57.5-64.5].
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