+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDFA4 |
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Sample | Insulin glargine (Toujeo®), oligomeric composition
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Citation | Date: 2019 Jul Title: The quaternary structure of insulin glargine and glulisine under formulation conditions Authors: Nagel N / Graewert M / Gao M / Heyse W / Jeffries C / Svergun D |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #2924 | Type: atomic / Symmetry: Dimer / Chi-square value: 2.30 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #2925 | Type: atomic / Symmetry: Hexamer / Chi-square value: 2.30 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Insulin glargine (Toujeo®), oligomeric composition / Specimen concentration: 10.91 mg/ml |
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Buffer | Name: Toujeo Fromulation (190 ug Zinc chloride, 2.7 mg m-Cresol, 20 mg glycerol 85%) pH: 4 |
Entity #1568 | Type: protein / Description: Insulin glargine (Toujeo®) / Formula weight: 6.051 / Num. of mol.: 6 Sequence: GIVEQCCTSI CSLYQLENYC GFVNQHLCGS HLVEALYLVC GERGFFYTPK TRR |
-Experimental information
Beam | Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 1.241 Å / Dist. spec. to detc.: 3.1 mm | |||||||||||||||||||||||||||||
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Detector | Name: Pilatus 6M | |||||||||||||||||||||||||||||
Scan | Title: Insulin glargine (Toujeo®), oligomeric composition / Measurement date: Jul 5, 2019 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 0.045 sec. / Number of frames: 20 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 933 /
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Result | Type of curve: single_conc Comments: Here, Toujeo measured at 10.91 mg/ml is displayed (formulation concentration). Measurements from 2 dilutions have been deposited in addition. Mixture analysis of the scattering profiles was ...Comments: Here, Toujeo measured at 10.91 mg/ml is displayed (formulation concentration). Measurements from 2 dilutions have been deposited in addition. Mixture analysis of the scattering profiles was performed with the program Oligomer. Data can be fit with a stable equilibrium of ~ 60 % hexamers (based on 1ev3.pdb) and ~ 40 % dimers. For higher concentration a strong influence of interparticle interference is observed, so that data at low s (>0.05 nm-1) is omitted for the fit. The presence of 3% dodecamers (based on 3W80.pdb) slightly improves the fit.
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