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- SASDFA4: Insulin glargine (Toujeo®), oligomeric composition -

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Basic information

Entry
Database: SASBDB / ID: SASDFA4
SampleInsulin glargine (Toujeo®), oligomeric composition
  • Insulin glargine (Toujeo®) (protein)
CitationDate: 2019 Jul
Title: The quaternary structure of insulin glargine and glulisine under formulation conditions
Authors: Nagel N / Graewert M / Gao M / Heyse W / Jeffries C / Svergun D
Contact author
  • Melissa Graewert (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

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MolmilJmol/JSmol

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Models

Model #2924
Type: atomic / Symmetry: Dimer / Chi-square value: 2.30
Search similar-shape structures of this assembly by Omokage search (details)
Model #2925
Type: atomic / Symmetry: Hexamer / Chi-square value: 2.30
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Insulin glargine (Toujeo®), oligomeric composition / Specimen concentration: 10.91 mg/ml
BufferName: Toujeo Fromulation (190 ug Zinc chloride, 2.7 mg m-Cresol, 20 mg glycerol 85%)
pH: 4
Entity #1568Type: protein / Description: Insulin glargine (Toujeo®) / Formula weight: 6.051 / Num. of mol.: 6
Sequence:
GIVEQCCTSI CSLYQLENYC GFVNQHLCGS HLVEALYLVC GERGFFYTPK TRR

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / : Germany / Type of source: X-ray synchrotron / Wavelength: 1.241 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 6M
Scan
Title: Insulin glargine (Toujeo®), oligomeric composition / Measurement date: Jul 5, 2019 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 0.045 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.1723 3.0419
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 933 /
MinMax
Q0.470276 3.04192
P(R) point1 933
R0 6.22
Result
Type of curve: single_conc
Comments: Here, Toujeo measured at 10.91 mg/ml is displayed (formulation concentration). Measurements from 2 dilutions have been deposited in addition. Mixture analysis of the scattering profiles was ...Comments: Here, Toujeo measured at 10.91 mg/ml is displayed (formulation concentration). Measurements from 2 dilutions have been deposited in addition. Mixture analysis of the scattering profiles was performed with the program Oligomer. Data can be fit with a stable equilibrium of ~ 60 % hexamers (based on 1ev3.pdb) and ~ 40 % dimers. For higher concentration a strong influence of interparticle interference is observed, so that data at low s (>0.05 nm-1) is omitted for the fit. The presence of 3% dodecamers (based on 3W80.pdb) slightly improves the fit.
ExperimentalStandardStandard error
MW35 kDa35 kDa194

P(R)GuinierGuinier error
Forward scattering, I0692.9 658.24 3.72
Radius of gyration, Rg1.9 nm1.8 nm0.1

MinMax
D-6.2
Guinier point1 -

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