ジャーナル: Sci Rep / 年: 2015 タイトル: Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions. 著者: Melissa A Graewert / Daniel Franke / Cy M Jeffries / Clement E Blanchet / Darja Ruskule / Katja Kuhle / Antje Flieger / Bernd Schäfer / Bernd Tartsch / Rob Meijers / Dmitri I Svergun / 要旨: Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed ...Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).