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TitleAutomated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions.
Journal, issue, pagesSci Rep, Vol. 5, Page 10734, Year 2015
Publish dateJun 1, 2015
AuthorsMelissa A Graewert / Daniel Franke / Cy M Jeffries / Clement E Blanchet / Darja Ruskule / Katja Kuhle / Antje Flieger / Bernd Schäfer / Bernd Tartsch / Rob Meijers / Dmitri I Svergun /
PubMed AbstractSmall angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed ...Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg).
External linksSci Rep / PubMed:26030009 / PubMed Central
MethodsSAS (X-ray synchrotron)
Structure data

SASDA97:
PlaB
Method: SAXS/SANS

Source
  • Legionella pneumophila (bacteria)

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