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| タイトル | Automated pipeline for purification, biophysical and x-ray analysis of biomacromolecular solutions. |
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| ジャーナル・号・ページ | Sci Rep, Vol. 5, Page 10734, Year 2015 |
| 掲載日 | 2015年6月1日 |
著者 | Melissa A Graewert / Daniel Franke / Cy M Jeffries / Clement E Blanchet / Darja Ruskule / Katja Kuhle / Antje Flieger / Bernd Schäfer / Bernd Tartsch / Rob Meijers / Dmitri I Svergun / ![]() |
| PubMed 要旨 | Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed ...Small angle X-ray scattering (SAXS), an increasingly popular method for structural analysis of biological macromolecules in solution, is often hampered by inherent sample polydispersity. We developed an all-in-one system combining in-line sample component separation with parallel biophysical and SAXS characterization of the separated components. The system coupled to an automated data analysis pipeline provides a novel tool to study difficult samples at the P12 synchrotron beamline (PETRA-3, EMBL/DESY, Hamburg). |
リンク | Sci Rep / PubMed:26030009 / PubMed Central |
| 手法 | SAS (X-ray synchrotron) |
| 構造データ | ![]() SASDA97: |
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