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Yorodumi- SASDA27: IcsS, IscU and CyaY dimeric complex (Cysteine desulfurase IscS +... -
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-Basic information
Entry | Database: SASBDB / ID: SASDA27 |
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Sample | IcsS, IscU and CyaY dimeric complex
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Function / homology | Function and homology information oxazole or thiazole biosynthetic process / IscS-TusA complex / tRNA 4-thiouridine biosynthesis / IscS-IscU complex / sulfur compound transport / selenocysteine catabolic process / sulfurtransferase complex / detection of UV / sulfur carrier activity / L-cysteine desulfurase complex ...oxazole or thiazole biosynthetic process / IscS-TusA complex / tRNA 4-thiouridine biosynthesis / IscS-IscU complex / sulfur compound transport / selenocysteine catabolic process / sulfurtransferase complex / detection of UV / sulfur carrier activity / L-cysteine desulfurase complex / selenocysteine lyase activity / tRNA wobble position uridine thiolation / cysteine desulfurase / cysteine desulfurase activity / L-cysteine catabolic process / thiamine biosynthetic process / [2Fe-2S] cluster assembly / iron-sulfur cluster assembly / ferric iron binding / ferrous iron binding / 2 iron, 2 sulfur cluster binding / pyridoxal phosphate binding / 4 iron, 4 sulfur cluster binding / intracellular iron ion homeostasis / copper ion binding / zinc ion binding / identical protein binding / metal ion binding / cytosol / cytoplasm Similarity search - Function |
Biological species | Escherichia coli (E. coli) |
Citation | Journal: Nat Commun / Year: 2010 Title: Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Authors: Filippo Prischi / Petr V Konarev / Clara Iannuzzi / Chiara Pastore / Salvatore Adinolfi / Stephen R Martin / Dmitri I Svergun / Annalisa Pastore / Abstract: Reduced levels of frataxin, an essential protein of as yet unknown function, are responsible for causing the neurodegenerative pathology Friedreich's ataxia. Independent reports have linked frataxin ...Reduced levels of frataxin, an essential protein of as yet unknown function, are responsible for causing the neurodegenerative pathology Friedreich's ataxia. Independent reports have linked frataxin to iron-sulphur cluster assembly through interactions with the two central components of this machinery: desulphurase Nfs1/IscS and the scaffold protein Isu/IscU. In this study, we use a combination of biophysical methods to define the structural bases of the interaction of CyaY (the bacterial orthologue of frataxin) with the IscS/IscU complex. We show that CyaY binds IscS as a monomer in a pocket between the active site and the IscS dimer interface. Recognition does not require iron and occurs through electrostatic interactions of complementary charged residues. Mutations at the complex interface affect the rates of enzymatic cluster formation. CyaY binding strengthens the affinity of the IscS/IscU complex. Our data suggest a new paradigm for understanding the role of frataxin as a regulator of IscS functions. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDA27 |
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-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data |
-External links
Related items in Molecule of the Month |
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-Models
Model #233 | Type: atomic / Software: SASREF / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Chi-square value: 4.9729 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #234 | Type: dummy / Software: DAMMIF / Radius of dummy atoms: 4.30 A / Symmetry: P2 / Chi-square value: 1.471369 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: IcsS, IscU and CyaY dimeric complex / Specimen concentration: 5 mg/ml / Entity id: 135 / 138 / 139 |
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Buffer | Name: Tris-HCl / Concentration: 20.00 mM / pH: 8 / Composition: 150 mM NaCl and 10 mM β-mercaptoethanol |
Entity #135 | Type: protein / Description: Cysteine desulfurase IscS / Formula weight: 43.597 / Num. of mol.: 2 / Source: Escherichia coli / References: UniProt: P0A6B7 Sequence: KLPIYLDYSA TTPVDPRVAE KMMQFMTMDG TFGNPASRSH RFGWQAEEAV DIARNQIADL VGADPREIVF TSGATESDNL AIKGAANFYQ KKGKHIITSK TEHKAVLDTC RQLEREGFEV TYLAPQRNGI IDLKELEAAM RDDTILVSIM HVNNEIGVVQ DIAAIGEMCR ...Sequence: KLPIYLDYSA TTPVDPRVAE KMMQFMTMDG TFGNPASRSH RFGWQAEEAV DIARNQIADL VGADPREIVF TSGATESDNL AIKGAANFYQ KKGKHIITSK TEHKAVLDTC RQLEREGFEV TYLAPQRNGI IDLKELEAAM RDDTILVSIM HVNNEIGVVQ DIAAIGEMCR ARGIIYHVDA TQSVGKLPID LSQLKVDLMS FSGHKIYGPK GIGALYVRRK PRVRIEAQMH GGGHERGMRS GTLPVHQIVG MGEAYRIAKE EMATEMERLR GLRNRLWNGI KDIEEVYLNG DLEHGAPNIL NVSFNYVEGE SLIMALKDLA VSSGSACTSA SLEPSYVLRA LGLNDELAHS SIRFSLGRFT TEEEIDYTIE LVRKSIGRLR DLSPLWEMYK Q |
Entity #138 | Type: protein Description: Iron-sulfur cluster assembly scaffold protein IscU Formula weight: 14.464 / Num. of mol.: 2 / Source: Escherichia coli / References: UniProt: P0ACD4 Sequence: MAYSEKVIDH YENPRNVGSL DKKDSNVGTG MVGAPACGDV MQLQIKVDDN GIIEDAKFKT YGCGSAIASS SLITEWVKGK SLEEAGAIKN SQIAEELELP PVKVHCSILA EDAIKAAIAD YKAKQGLEHH HHHH |
Entity #139 | Type: protein / Description: Protein CyaY / Formula weight: 12.231 / Num. of mol.: 2 / Source: Escherichia coli / References: UniProt: P27838 Sequence: MNDSEFHRLA DQLWLTIEER LDDWDGDSDI DCEINGGVLT ITFENGSKII INRQEPLHQV WLATKQGGYH FDLKGDEWIC DRSGETFWDL LEQAATQQAG ETVSFR |
-Experimental information
Beam | Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Shape: 0.6 / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 2.7 mm | ||||||||||||||||||
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Detector | Name: Pilatus 1M-W / Pixsize x: 0.172 mm | ||||||||||||||||||
Scan | Title: suy / Measurement date: Nov 5, 2009 / Storage temperature: 15 °C / Cell temperature: 15 °C / Exposure time: 30 sec. / Number of frames: 4 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 4.5a / Number of points: 512 /
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Result | D max: 11.93 / Type of curve: single_conc / Standard: BSA Comments: Reduced levels of frataxin, an essential protein of as yet unknown function, are responsible for causing the neurodegenerative pathology Friedreich’s ataxia. Independent reports have ...Comments: Reduced levels of frataxin, an essential protein of as yet unknown function, are responsible for causing the neurodegenerative pathology Friedreich’s ataxia. Independent reports have linked frataxin to iron–sulphur cluster assembly through interactions with the two central components of this machinery: desulphurase Nfs1/IscS and the scaffold protein Isu/IscU. In this study, we use a combination of biophysical methods to define the structural bases of the interaction of CyaY (the bacterial orthologue of frataxin) with the IscS/IscU complex. We show that CyaY binds IscS as a monomer in a pocket between the active site and the IscS dimer interface. Recognition does not require iron and occurs through electrostatic interactions of complementary charged residues. Mutations at the complex interface affect the rates of enzymatic cluster formation. CyaY binding strengthens the affinity of the IscS/IscU complex. Our data suggest a new paradigm for understanding the role of frataxin as a regulator of IscS functions.
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