SASDAS6: Plectin, fragment of the plakin domain encompassing the spectrin ...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAS6

試料

Plectin, fragment of the plakin domain encompassing the spectrin repeats SR3-SR4-SR5 and the SH3

• Plectin (protein), Homo sapiens

機能・相同性

分子機能:

protein-containing complex organization / actomyosin contractile ring assembly actin filament organization / skeletal myofibril assembly / tight junction organization / Type I hemidesmosome assembly / hemidesmosome assembly / hemidesmosome / leukocyte migration involved in immune response / intermediate filament organization / : / regulation of vascular permeability / intermediate filament cytoskeleton organization / dystroglycan binding / cellular response to hydrostatic pressure / fibroblast migration / costamere / T cell chemotaxis / cellular response to fluid shear stress / peripheral nervous system myelin maintenance / cardiac muscle cell development / adherens junction organization / myoblast differentiation / intermediate filament cytoskeleton / response to food / structural constituent of muscle / ankyrin binding / intermediate filament / Assembly of collagen fibrils and other multimeric structures / sarcomere organization / nucleus organization / keratinocyte development / transmission of nerve impulse / brush border / sarcoplasm / Caspase-mediated cleavage of cytoskeletal proteins / establishment of skin barrier / skeletal muscle fiber development / respiratory electron transport chain / mitochondrion organization / wound healing / cell morphogenesis / protein localization / multicellular organism growth / structural constituent of cytoskeleton / sarcolemma / Z disc / cellular response to mechanical stimulus / : / actin filament binding / myelin sheath / gene expression / mitochondrial outer membrane / cadherin binding / axon / focal adhesion / dendrite / perinuclear region of cytoplasm / RNA binding / extracellular exosome / plasma membrane / cytoplasm / cytosol

ドメイン・相同性:

: / Spectrin-like repeat / Spectrin repeat / Spectrin-like repeat / Desmoplakin, spectrin-like domain / Spectrin like domain / Plectin repeat / Plectin repeat / Plakin repeat superfamily / Desmoplakin, SH3 domain / SH3 domain / Plectin repeat / Plakin / Spectrin/alpha-actinin / Actinin-type actin-binding domain signature 1. / Actinin-type actin-binding domain signature 2. / Spectrin repeats / Actinin-type actin-binding domain, conserved site / Calponin homology domain / Calponin homology (CH) domain / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / Plectin/S10, N-terminal / Plectin/S10 domain / Src homology 3 (SH3) domain profile. / SH3 domain / Winged helix-like DNA-binding domain superfamily

構成要素:

Plectin

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: J Biol Chem / 年: 2011; タイトル: The structure of the plakin domain of plectin reveals a non-canonical SH3 domain interacting with its fourth spectrin repeat.; 著者: Esther Ortega / Rubén M Buey / Arnoud Sonnenberg / José M de Pereda / ; 要旨: Plectin belongs to the plakin family of cytoskeletal crosslinkers, which is part of the spectrin superfamily. Plakins contain an N-terminal conserved region, the plakin domain, which is formed by an array of spectrin repeats (SR) and a Src-homology 3 (SH3), and harbors binding sites for junctional proteins. We have combined x-ray crystallography and small angle x-ray scattering (SAXS) to elucidate the structure of the central region of the plakin domain of plectin, which corresponds to the SR3, SR4, SR5, and SH3 domains. The crystal structures of the SR3-SR4 and SR4-SR5-SH3 fragments were determined to 2.2 and 2.95 Å resolution, respectively. The SH3 of plectin presents major alterations as compared with canonical Pro-rich binding SH3 domains, suggesting that plectin does not recognize Pro-rich motifs. In addition, the SH3 binding site is partially occluded by an intramolecular contact with the SR4. Residues of this pseudo-binding site and the SR4/SH3 interface are conserved within the plakin family, suggesting that the structure of this part of the plectin molecule is similar to that of other plakins. We have created a model for the SR3-SR4-SR5-SH3 region, which agrees well with SAXS data in solution. The three SRs form a semi-flexible rod that is not altered by the presence of the SH3 domain, and it is similar to those found in spectrins. The flexibility of the plakin domain, in analogy with spectrins, might contribute to the role of plakins in maintaining the stability of tissues subject to mechanical stress.

登録者

• Jose M de Pereda (USAL, La Universidad de Salamanca, Salamanca, Spain)

モデル

• モデル #218: ; タイプ: dummy / ソフトウェア: DAMMIF / ダミー原子の半径: 3.00 A / カイ2乗値: 1.48328041; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Plectin, fragment of the plakin domain encompassing the spectrin repeats SR3-SR4-SR5 and the SH3; 試料濃度: 1.20-9.60
• バッファ: 名称: Sodium Phosphate / 濃度: 20.00 mM / pH: 7.5 / 組成: 150 mM NaCl, 5% glycerol, 2.5 mM DTT

要素 #132

タイプ: protein / 記述: Plectin / 分子量: 42.896 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: Q15149
配列:

ELEDSTLRYL QDLLAWVEEN QHRVDGAEWG VDLPSVEAQL GSHRGLHQSI EEFRAKIERA RSDEGQLSPA TRGAYRDCLG RLDLQYAKLL NSSKARLRSL ESLHSFVAAA TKELMWLNEK EEEEVGFDWS DRNTNMTAKK ESYSALMREL ELKEKKIKEL QNAGDRLLRE DHPARPTVES FQAALQTQWS WMLQLCCCIE AHLKENAAYF QFFSDVREAE GQLQKLQEAL RRKYSCDRSA TVTRLEDLLQ DAQDEKEQLN EYKGHLSGLA KRAKAVVQLK PRHPAHPMRG RLPLLAVCDY KQVEVTVHKG DECQLVGPAQ PSHWKVLSSS GSEAAVPSVC FLVPPPNQEA QEAVTRLEAQ HQALVTLWHQ LHVDMK

実験情報

• ビーム: 設備名称: Swiss Light Source cSAXS / 地域: Villigen / 国: Switzerland / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 2.15 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Plectin / 測定日: 2009年7月24日 / セル温度: 10 °C / 照射時間: 0.5 sec. / フレーム数: 60 / 単位: 1/nm /

	Min	Max
Q	0.1709	3.5044

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 280 /

	Min	Max
Q	0.2136	3.011
P(R) point	8	287
R	0	14.5

結果

D max: 14.5 / カーブのタイプ: extrapolated
コメント: Use of SAXS to analyze the structure of central region of the plakin domain of plectin.

	実験値	Porod
分子量	37.7 kDa	33 kDa
体積	-	57 nm3

	P(R)	Guinier
前方散乱 I0	22.4	23.7
慣性半径, Rg	4.2 nm	4.4 nm