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Yorodumi- SASDCJ9: Bifunctional enzyme responsible for the synthesis and hydrolysis ... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDCJ9 |
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Sample | Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) without GDP
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Function / homology | Function and homology information |
Biological species | Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) (bacteria) |
Citation | Date: 2018 Apr 13 Title: The GDP-switched GAF domain of DcpA modulates the concerted synthesis/hydrolysis of c-di-GMP in Mycobacterium smegmatis Authors: Chen H / Li N / Luo Y / Jiang Y / Zhou C / Chen Y |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDCJ9 |
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-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data |
-External links
Related items in Molecule of the Month |
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-Models
Model #1641 | Type: atomic / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Chi-square value: 2.26 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #1632 | Type: dummy / Radius of dummy atoms: 5.25 A / Chi-square value: 19.689 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) without GDP Specimen concentration: 5 mg/ml |
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Buffer | Name: 20 mM HEPES, 100 mM NaCl, 5% glycerol, 2 mM MgCl2 / pH: 7.5 |
Entity #876 | Type: protein / Description: Sensory box/response regulator / Formula weight: 67.758 / Num. of mol.: 2 Source: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) References: UniProt: A0QUG3 Sequence: MGHHHHHHMM SESLDVLVTS VATQLMAVEA ATSVSVSQQV LAELVSFFDV DVSFLRHNDH RAHATRLVAE WPPRPVDAAT DPIAVVHFAD ADPVFAMAEH LKEPAVFRPE PLYDDYQRTI EAGRHIPATS MACVPLLSGD VTTGVLGFVK FGDREWLPAE LNALKAIASL ...Sequence: MGHHHHHHMM SESLDVLVTS VATQLMAVEA ATSVSVSQQV LAELVSFFDV DVSFLRHNDH RAHATRLVAE WPPRPVDAAT DPIAVVHFAD ADPVFAMAEH LKEPAVFRPE PLYDDYQRTI EAGRHIPATS MACVPLLSGD VTTGVLGFVK FGDREWLPAE LNALKAIASL FAQVQARIEA EERLRYLADH DHLTGLYNRR ALMAHLEARL APGQPGPVAV MFFDLDRLKA INDYLGHTAG DAFISILAQR LQRGDDAPKL IARLGGDEFV VVPDDPMSLD EATALAYRLQ SVLRERVTVD GEMLTRTVSI GIAQGIPGKD STSDVLNRAD HAVLTAKGSG GNRVAVFSDA MAMEIDFRND IELHLQSVIE GGALVLHYLP EIDMRTGEVL AAEALVRWEH PTRGLLSPDS FIGVAESINL AGELGRWVLR TACAEFSRWR ANGVGRNIVL RINVSPVQLV TDGFVESVAG IMKEFGLPRG SVCLEITESV VVQDIETTRT TLTGLHNVGV QVAIDDFGTG YSVLSLLKSL PVDTLKIDRS FVAELGSNPG DLPIVRAVIA LAGAFGLQLV AEGVETERAA LTLLRHGCYR AQGFLLSKPI LGSEMQTLLA KGRVPVHFSA APRM |
-Experimental information
Beam | Instrument name: Shanghai Synchrotron Radiation Facility (SSRF) BL19U2 City: Shanghai / 国: China / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 2.25 mm | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
Scan | Title: Bifunctional enzyme responsible for the synthesis and hydrolysis of c-di-GMP (DcpA) without GDP Measurement date: Jun 26, 2016 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 20 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 4.6 / Number of points: 299 /
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Result | Type of curve: single_conc /
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