+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDCU7 |
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試料 | TRIP18(7.5R)SN-f5
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生物種 | synthetic construct (人工物) |
引用 | ジャーナル: Nat Biotechnol / 年: 2017 タイトル: Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo. 著者: Ajasja Ljubetič / Fabio Lapenta / Helena Gradišar / Igor Drobnak / Jana Aupič / Žiga Strmšek / Duško Lainšček / Iva Hafner-Bratkovič / Andreja Majerle / Nuša Krivec / Mojca Benčina ...著者: Ajasja Ljubetič / Fabio Lapenta / Helena Gradišar / Igor Drobnak / Jana Aupič / Žiga Strmšek / Duško Lainšček / Iva Hafner-Bratkovič / Andreja Majerle / Nuša Krivec / Mojca Benčina / Tomaž Pisanski / Tanja Ćirković Veličković / Adam Round / José María Carazo / Roberto Melero / Roman Jerala / 要旨: Polypeptides and polynucleotides are natural programmable biopolymers that can self-assemble into complex tertiary structures. We describe a system analogous to designed DNA nanostructures in which ...Polypeptides and polynucleotides are natural programmable biopolymers that can self-assemble into complex tertiary structures. We describe a system analogous to designed DNA nanostructures in which protein coiled-coil (CC) dimers serve as building blocks for modular de novo design of polyhedral protein cages that efficiently self-assemble in vitro and in vivo. We produced and characterized >20 single-chain protein cages in three shapes-tetrahedron, four-sided pyramid, and triangular prism-with the largest containing >700 amino-acid residues and measuring 11 nm in diameter. Their stability and folding kinetics were similar to those of natural proteins. Solution small-angle X-ray scattering (SAXS), electron microscopy (EM), and biophysical analysis confirmed agreement of the expressed structures with the designs. We also demonstrated self-assembly of a tetrahedral structure in bacteria, mammalian cells, and mice without evidence of inflammation. A semi-automated computational design platform and a toolbox of CC building modules are provided to enable the design of protein cages in any polyhedral shape. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #1584 | タイプ: atomic / ソフトウェア: (9.16) / ダミー原子の半径: 1.90 A コメント: The model contributes 76% to the observed scattering curve. カイ2乗値: 0.96 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #1585 | タイプ: atomic / ソフトウェア: (9.16) / ダミー原子の半径: 1.90 A コメント: The model contributes 24% to the observed scattering curve. カイ2乗値: 0.96 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: TRIP18(7.5R)SN-f5 / 試料濃度: 0.40-4.10 |
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バッファ | 名称: 20 mM Tris 150 mM NaCl 10% glycerol / pH: 7.5 |
要素 #807 | 名称: TRIP18SN / タイプ: protein / 記述: TRIP18(7.5R)SN-f5 / 分子量: 81.866 / 分子数: 1 / 由来: synthetic construct 配列: MSPEDENSQL EEKISQLKQK NSELKEEIQQ LEYGSGPGLE QIEERLEQIE ERLQAKEWEK AQLREELQAL REKLAQLGSG PGSPEDEIRQ LEQENSQLER ENQRLEQEIY QLERGSGPGS PEDKNSELKE EIQQLEEENQ QLEEKISELK YGSGPGSRMK QLEDKVEELL ...配列: MSPEDENSQL EEKISQLKQK NSELKEEIQQ LEYGSGPGLE QIEERLEQIE ERLQAKEWEK AQLREELQAL REKLAQLGSG PGSPEDEIRQ LEQENSQLER ENQRLEQEIY QLERGSGPGS PEDKNSELKE EIQQLEEENQ QLEEKISELK YGSGPGSRMK QLEDKVEELL SKNYHLENEV ERLKKLVGSG PGLEEELKQL EEELQAIEEQ LAQLQWKAQA RKEKLAQLKE KLGSGPGSPE DENQSLEQKN SQLKQEISQL EQEIQQLEYG SGPGLEQIEE RLEQIEERLQ AKEWEKAQLR EELQALREKL AQLGSGPGSR MKQLEDKVEE LLSKNYHLEN EVERLKKLVG SGPGSPEDEI QQLEEEISQL EQKNSELKEK NQELKYGSGP GSPEDENQSL EQEISQLEQE IQQLEQKNSE LKYGSGPGSP EDKNSQLKEE NSQLEEKIEQ LKEKIQELKY GSGPGSPEDK IEELKEKNSQ LKEKNEELKQ KIYELKEGSG PGDIEQELER AKESIRRLEQ EVNQERSRMQ YLQTLLEKGS GPGSPEDKNE QLKEKISELK EKIEQLKEEN QSLEYGSGPG LEEELKQLEE ELQAIEEQLA QLQWKAQARK EKLAQLKEKL GSGPGSPEDK ISQLKEKIQQ LKQENQQLEE ENSQLEYGSG PGDIEQELER AKESIRRLEQ EVNQERSRMQ YLQTLLEKLE HHHHHHHH |
-実験情報
ビーム | 設備名称: PETRA III P12 / 地域: Hamburg / 国: Germany / 形状: Point / 線源: X-ray synchrotron / 波長: 0.124 Å / スペクトロメータ・検出器間距離: 2 mm | ||||||||||||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M-W / Pixsize x: 0.172 mm | ||||||||||||||||||||||||||||||||||||||||||
スキャン | タイトル: TRIP18(7.5R)SN-f5 / 測定日: 2016年6月10日 / 保管温度: 10 °C / セル温度: 20 °C / 照射時間: 0.045 sec. / フレーム数: 20 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 342 /
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結果 | カーブのタイプ: merged
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