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Yorodumi- SASDCP7: Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A ... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDCP7 |
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Sample | Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L SUMO fusion protein
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Biological species | Mus musculus (house mouse) |
Citation | Journal: Structure / Year: 2018 Title: Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Authors: Andrew W Malaby / Sanchaita Das / Srinivas Chakravarthy / Thomas C Irving / Osman Bilsel / David G Lambright / Abstract: Membrane dynamic processes including vesicle biogenesis depend on Arf guanosine triphosphatase (GTPase) activation by guanine nucleotide exchange factors (GEFs) containing a catalytic Sec7 domain and ...Membrane dynamic processes including vesicle biogenesis depend on Arf guanosine triphosphatase (GTPase) activation by guanine nucleotide exchange factors (GEFs) containing a catalytic Sec7 domain and a membrane-targeting module such as a pleckstrin homology (PH) domain. The catalytic output of cytohesin family Arf GEFs is controlled by autoinhibitory interactions that impede accessibility of the exchange site in the Sec7 domain. These restraints can be relieved through activator Arf-GTP binding to an allosteric site comprising the PH domain and proximal autoinhibitory elements (Sec7-PH linker and C-terminal helix). Small-angle X-ray scattering and negative-stain electron microscopy were used to investigate the structural organization and conformational dynamics of cytohesin-3 (Grp1) in autoinhibited and active states. The results support a model in which hinge dynamics in the autoinhibited state expose the activator site for Arf-GTP binding, while subsequent C-terminal helix unlatching and repositioning unleash conformational entropy in the Sec7-PH linker to drive exposure of the exchange site. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #1559 | Type: dummy / Software: (r2835) / Radius of dummy atoms: 3.10 A / Symmetry: P1 / Comment: Most representative of 100 dammif models / Chi-square value: 1.565001 / P-value: 0.000114 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #1560 | Type: dummy / Radius of dummy atoms: 3.00 A / Symmetry: P1 Comment: Filtered average of 10 filtered averages of 10 dammif models Chi-square value: 1.565001 / P-value: 0.000114 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L SUMO fusion protein Specimen concentration: 2.6 mg/ml |
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Buffer | Name: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate pH: 8 |
Entity #797 | Type: protein Description: Grp1 63-399 E161A 6GS Arf6 Q67L SUMO fusion protein Formula weight: 72.469 / Num. of mol.: 1 / Source: Mus musculus Sequence: MGHHHHHHGS TTQRNKQIAM GRKKFNMDPK KGIQFLIEND LLQSSPEDVA QFLYKGEGLN KTVIGDYLGE RDEFNIKVLQ AFVELHEFAD LNLVQALRQF LWSFRLPGEA QKIDRMMEAF ASRYCLCNPG VFQSTDTCYV LSFAIIMLNT SLHNHNVRDK PTAERFIAMN ...Sequence: MGHHHHHHGS TTQRNKQIAM GRKKFNMDPK KGIQFLIEND LLQSSPEDVA QFLYKGEGLN KTVIGDYLGE RDEFNIKVLQ AFVELHEFAD LNLVQALRQF LWSFRLPGEA QKIDRMMEAF ASRYCLCNPG VFQSTDTCYV LSFAIIMLNT SLHNHNVRDK PTAERFIAMN RGINEGGDLP EELLRNLYES IKNEPFKIPE DDGNDLTHTF FNPDREGWLL KLGGRVKTWK RRWFILTDNC LYYFEYTTDK EPRGIIPLEN LSIREVEDPR KPNCFELYNP SHKGQVIKAC KTEADGRVVE GNHVVYRISA PSPEEKEEWM KSIKASISRD PFYDMLATRK RRIANKKGSG SGSGSGSGSM GKVLSKIFGN KEMRILMLGL DAAGKTTILY KLKLGQSVTT IPTVGFNVET VTYKNVKFNV WDVGGQDKIR PLWRHYYTGT QGLIFVVDCA DRDRIDEARQ ELHRIINDRE MRDAIILIFA NKQDLPDAMK PHEIQEKLGL TRIRDRNWYV QPSCATSGDG LYEGLTWLTS NYKSGDSEVN QEAKPEVKPE VKPETHINLK VSDGSSEIFF KIKKTTPLRR LMEAFAKRQG KEMDSLRFLY DGIRIQADQT PEDLDMEDND IIEAHREQIG GS |
-Experimental information
Beam | Instrument name: Advanced Photon Source (APS) BioCAT 18ID / City: Argonne, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.10332 Å / Dist. spec. to detc.: 2.5 mm | ||||||||||||||||||||||||||||||||||||
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Detector | Name: MAR 165 CCD | ||||||||||||||||||||||||||||||||||||
Scan | Title: Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L SUMO fusion protein Measurement date: Nov 15, 2013 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 1 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 77 /
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Result | Type of curve: other /
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