+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDCJ6 |
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試料 | MmoQ Response regulator (fragment 20-298) from Methylococcus capsulatus str. Bath, Northeast Structural Genomics Consortium Target McR175G
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機能・相同性 | 機能・相同性情報 diguanylate cyclase / negative regulation of bacterial-type flagellum-dependent cell motility / diguanylate cyclase activity / cell adhesion involved in single-species biofilm formation / phosphorelay signal transduction system / plasma membrane 類似検索 - 分子機能 |
生物種 | Methylococcus capsulatus (バクテリア) |
引用 | ジャーナル: Biopolymers / 年: 2011 タイトル: Small angle X-ray scattering as a complementary tool for high-throughput structural studies. 著者: Thomas D Grant / Joseph R Luft / Jennifer R Wolfley / Hiro Tsuruta / Anne Martel / Gaetano T Montelione / Edward H Snell / 要旨: Structural crystallography and nuclear magnetic resonance (NMR) spectroscopy are the predominant techniques for understanding the biological world on a molecular level. Crystallography is constrained ...Structural crystallography and nuclear magnetic resonance (NMR) spectroscopy are the predominant techniques for understanding the biological world on a molecular level. Crystallography is constrained by the ability to form a crystal that diffracts well and NMR is constrained to smaller proteins. Although powerful techniques, they leave many soluble, purified structurally uncharacterized protein samples. Small angle X-ray scattering (SAXS) is a solution technique that provides data on the size and multiple conformations of a sample, and can be used to reconstruct a low-resolution molecular envelope of a macromolecule. In this study, SAXS has been used in a high-throughput manner on a subset of 28 proteins, where structural information is available from crystallographic and/or NMR techniques. These crystallographic and NMR structures were used to validate the accuracy of molecular envelopes reconstructed from SAXS data on a statistical level, to compare and highlight complementary structural information that SAXS provides, and to leverage biological information derived by crystallographers and spectroscopists from their structures. All the ab initio molecular envelopes calculated from the SAXS data agree well with the available structural information. SAXS is a powerful albeit low-resolution technique that can provide additional structural information in a high-throughput and complementary manner to improve the functional interpretation of high-resolution structures. |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #1437 | タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 8.497225 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #1438 | タイプ: dummy / ダミー原子の半径: 2.00 A / カイ2乗値: 2.319529 Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: MmoQ Response regulator (fragment 20-298) from Methylococcus capsulatus str. Bath, Northeast Structural Genomics Consortium Target McR175G 試料濃度: 2.50-5.25 |
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バッファ | 名称: 5 mM DTT 100 mM NaCl 10 mM Tris-HCl 0.02 % NaN3 / pH: 7.5 |
要素 #753 | タイプ: protein / 記述: MmoQ / 分子量: 32.023 / 分子数: 1 / 由来: Methylococcus capsulatus / 参照: UniProt: Q7WZ31 配列: MDRWNMHKPM LCDSLPTASR TAAAILNLAQ REDVTAEALA QLIQTDPALT GRILRFANAP AQGTRRPVAS VIDAIDLVGL PAVRQFALSL SLIDAHREGR CEAFDYAAYW QKSLARAVAL QSITAQASTV APKEAFTLGL LADVGRLALA TAWPEEYSEC LRKADGEALI ...配列: MDRWNMHKPM LCDSLPTASR TAAAILNLAQ REDVTAEALA QLIQTDPALT GRILRFANAP AQGTRRPVAS VIDAIDLVGL PAVRQFALSL SLIDAHREGR CEAFDYAAYW QKSLARAVAL QSITAQASTV APKEAFTLGL LADVGRLALA TAWPEEYSEC LRKADGEALI ALERERFATD HDELTRMLLT DWGFPQVFID ALQLSQQDEI RDEGRTGRFA RQLALAQHIA DHRLAEEPRR AALSPLLRAE ARRCGLGDED LARLLADPPA DWLDWTRTIG LEHHHHHH |
-実験情報
ビーム | 設備名称: Stanford Synchrotron Radiation Lightsource (SSRL) BL4-2 地域: Stanford, CA / 国: USA / 線源: X-ray synchrotron / 波長: 0.13 Å / スペクトロメータ・検出器間距離: 1.5 mm | ||||||||||||||||||
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検出器 | 名称: Rayonix MX225-HE | ||||||||||||||||||
スキャン | タイトル: MmoQ Response regulator (fragment 20-298) from Methylococcus capsulatus str. Bath, Northeast Structural Genomics Consortium Target McR175G 測定日: 2010年2月12日 / 保管温度: -80 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 20 / 単位: 1/A /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.5a / ポイント数: 342 /
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結果 | D max: 8.2 / カーブのタイプ: single_conc /
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