+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDC45 |
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Sample | Alpha domain of autotransporter protein UpaB from UPEC
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Function / homology | Function and homology information |
Biological species | E. Coli CFT073 |
Citation | Journal: Nat Commun / Year: 2019 Title: Unique structural features of a bacterial autotransporter adhesin suggest mechanisms for interaction with host macromolecules. Authors: Jason J Paxman / Alvin W Lo / Matthew J Sullivan / Santosh Panjikar / Michael Kuiper / Andrew E Whitten / Geqing Wang / Chi-Hao Luan / Danilo G Moriel / Lendl Tan / Kate M Peters / Minh-Duy ...Authors: Jason J Paxman / Alvin W Lo / Matthew J Sullivan / Santosh Panjikar / Michael Kuiper / Andrew E Whitten / Geqing Wang / Chi-Hao Luan / Danilo G Moriel / Lendl Tan / Kate M Peters / Minh-Duy Phan / Christine L Gee / Glen C Ulett / Mark A Schembri / Begoña Heras / Abstract: Autotransporters are the largest family of outer membrane and secreted proteins in Gram-negative bacteria. Most autotransporters are localised to the bacterial surface where they promote colonisation ...Autotransporters are the largest family of outer membrane and secreted proteins in Gram-negative bacteria. Most autotransporters are localised to the bacterial surface where they promote colonisation of host epithelial surfaces. Here we present the crystal structure of UpaB, an autotransporter that is known to contribute to uropathogenic E. coli (UPEC) colonisation of the urinary tract. We provide evidence that UpaB can interact with glycosaminoglycans and host fibronectin. Unique modifications to its core β-helical structure create a groove on one side of the protein for interaction with glycosaminoglycans, while the opposite face can bind fibronectin. Our findings reveal far greater diversity in the autotransporter β-helix than previously thought, and suggest that this domain can interact with host macromolecules. The relevance of these interactions during infection remains unclear. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #1327 | Type: dummy / Software: (2.8.0) / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Chi-square value: 1.468 / P-value: 0.021000 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #1328 | Type: mix / Software: (1.1) / Radius of dummy atoms: 1.90 A / Symmetry: P1 Comment: Coral was used to model residues from the N-terminus and C-terminus missing from the PDB model Chi-square value: 1.77 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: Alpha domain of autotransporter protein UpaB from UPEC Specimen concentration: 0.10-2.70 |
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Buffer | Name: 25 mM HEPES 150 mM NaCl / pH: 7 |
Entity #708 | Name: Alpha-UpaB / Type: protein / Description: Alpha domain of autotransporter protein UpaB / Formula weight: 47.673 / Num. of mol.: 1 / Source: E. Coli CFT073 / References: UniProt: A0A0H2V5A3 Sequence: SNATDSTVST DPVTLNTEKT TLDQDVVING DNKITAVTIE TSDSDKDLNV TFGGHDITAA STVNQDFVEG VKVSGNKNVV INATDSTITA QGEGTYVRTA MVIDSTGDVV VNGGNFVAKN EKGSATGISL EATTGNNLTL NGTTINAQGN KSYSNGSTAI FAQKGNLLQG ...Sequence: SNATDSTVST DPVTLNTEKT TLDQDVVING DNKITAVTIE TSDSDKDLNV TFGGHDITAA STVNQDFVEG VKVSGNKNVV INATDSTITA QGEGTYVRTA MVIDSTGDVV VNGGNFVAKN EKGSATGISL EATTGNNLTL NGTTINAQGN KSYSNGSTAI FAQKGNLLQG FDGDATDNIT LADSNIINGG IETIVTAGNK TGIHTVNLNI KDGSVIGAAN NKQTIYASAS AQGAGSATQN LNLSVADSTI YSDVLALSES ENSASTTTNV NMNVARSYWE GNAYTFNSGD KAGSDLDINL SDSSVWKGKV SGAGDASVSL QNGSVWNVTG SSTVDALAVK DSTVNITKAT VNTGTFASQN GTLIVDASSE NTLDISGKAS GDLRVYSAGS LDLINEQTAF ISTGKDSTLK ATGTTEGGLY QYDLTQGADG NFYFVKNTHK ASNASSVIQA MAAAPANVAN LQADTL |
-Experimental information
Beam | Instrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / 国: Australia / Shape: Point / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å / Dist. spec. to detc.: 1.428 mm | ||||||||||||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||||||||||||||
Scan | Title: Alpha domain of autotransporter protein UpaB from UPEC Measurement date: May 1, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 35 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 4.6 / Number of points: 396 /
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Result | Type of curve: single_conc
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