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Structure paper

TitleUnique structural features of a bacterial autotransporter adhesin suggest mechanisms for interaction with host macromolecules.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 1967, Year 2019
Publish dateApr 29, 2019
AuthorsJason J Paxman / Alvin W Lo / Matthew J Sullivan / Santosh Panjikar / Michael Kuiper / Andrew E Whitten / Geqing Wang / Chi-Hao Luan / Danilo G Moriel / Lendl Tan / Kate M Peters / Minh-Duy Phan / Christine L Gee / Glen C Ulett / Mark A Schembri / Begoña Heras /
PubMed AbstractAutotransporters are the largest family of outer membrane and secreted proteins in Gram-negative bacteria. Most autotransporters are localised to the bacterial surface where they promote colonisation ...Autotransporters are the largest family of outer membrane and secreted proteins in Gram-negative bacteria. Most autotransporters are localised to the bacterial surface where they promote colonisation of host epithelial surfaces. Here we present the crystal structure of UpaB, an autotransporter that is known to contribute to uropathogenic E. coli (UPEC) colonisation of the urinary tract. We provide evidence that UpaB can interact with glycosaminoglycans and host fibronectin. Unique modifications to its core β-helical structure create a groove on one side of the protein for interaction with glycosaminoglycans, while the opposite face can bind fibronectin. Our findings reveal far greater diversity in the autotransporter β-helix than previously thought, and suggest that this domain can interact with host macromolecules. The relevance of these interactions during infection remains unclear.
External linksNat Commun / PubMed:31036849 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution1.97 Å
Structure data

SASDC45:
Alpha domain of autotransporter protein UpaB from UPEC
Method: SAXS/SANS

PDB-6bea:
Crystal structure of the autotransporter UpaB from E. coli strain CFT073
Method: X-RAY DIFFRACTION / Resolution: 1.97 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-SO4:
SULFATE ION

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

Source
  • Escherichia coli cft073 (bacteria)
  • escherichia coli o6:h1 (bacteria)
KeywordsPROTEIN BINDING / autotransporter protein / bacterial adhesin / virulence factor / fibronectin / glycosaminoglycan

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