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- PDB-8t31: Crystal structure of GABARAP in complex with the LIR of TP53INP2/DOR -

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Basic information

Entry
Database: PDB / ID: 8t31
TitleCrystal structure of GABARAP in complex with the LIR of TP53INP2/DOR
Components
  • Gamma-aminobutyric acid receptor-associated protein
  • Tumor protein p53-inducible nuclear protein 2
KeywordsPROTEIN BINDING / Autophagy / GABARAP selectivity / DOR LIR / TP53INP2 LIR
Function / homology
Function and homology information


negative regulation of protein localization / positive regulation of protein K48-linked ubiquitination / regulation of Rac protein signal transduction / tissue homeostasis / GABA receptor binding / phosphatidylethanolamine binding / TBC/RABGAPs / cellular response to nitrogen starvation / microtubule associated complex / Macroautophagy ...negative regulation of protein localization / positive regulation of protein K48-linked ubiquitination / regulation of Rac protein signal transduction / tissue homeostasis / GABA receptor binding / phosphatidylethanolamine binding / TBC/RABGAPs / cellular response to nitrogen starvation / microtubule associated complex / Macroautophagy / regulation of neurotransmitter receptor localization to postsynaptic specialization membrane / smooth endoplasmic reticulum / autophagosome membrane / axoneme / extrinsic apoptotic signaling pathway via death domain receptors / autophagosome assembly / autophagosome maturation / beta-tubulin binding / protein targeting / mitophagy / sperm midpiece / autophagosome / ubiquitin binding / PML body / GABA-ergic synapse / microtubule cytoskeleton organization / osteoblast differentiation / intracellular protein localization / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / protein transport / actin cytoskeleton / cytoplasmic vesicle / ubiquitin-dependent protein catabolic process / microtubule binding / chemical synaptic transmission / microtubule / lysosome / Golgi membrane / intracellular membrane-bounded organelle / ubiquitin protein ligase binding / positive regulation of DNA-templated transcription / nucleus / plasma membrane / cytosol
Similarity search - Function
Tumour protein p53-inducible nuclear protein / DOR family / Autophagy protein Atg8 ubiquitin-like / Autophagy protein Atg8 ubiquitin like / Ubiquitin-like domain superfamily
Similarity search - Domain/homology
Gamma-aminobutyric acid receptor-associated protein / Tumor protein p53-inducible nuclear protein 2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.096 Å
AuthorsAli, M.G.H. / Wahba, H.M. / Cyr, N. / Omichinski, J.G.
Funding support Canada, 1items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR) Canada
CitationJournal: Autophagy / Year: 2024
Title: Structural and functional characterization of the role of acetylation on the interactions of the human Atg8-family proteins with the autophagy receptor TP53INP2/DOR.
Authors: Ali, M.G. / Wahba, H.M. / Igelmann, S. / Cyr, N. / Ferbeyre, G. / Omichinski, J.G.
History
DepositionJun 7, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 22, 2024Provider: repository / Type: Initial release
Revision 1.1Jun 5, 2024Group: Database references / Category: citation / Item: _citation.page_first / _citation.page_last
Revision 1.2Sep 4, 2024Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Sep 11, 2024Group: Database references / Category: citation_author / Item: _citation_author.identifier_ORCID

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Gamma-aminobutyric acid receptor-associated protein
B: Tumor protein p53-inducible nuclear protein 2
C: Gamma-aminobutyric acid receptor-associated protein
D: Tumor protein p53-inducible nuclear protein 2
E: Gamma-aminobutyric acid receptor-associated protein
F: Tumor protein p53-inducible nuclear protein 2
G: Gamma-aminobutyric acid receptor-associated protein
H: Tumor protein p53-inducible nuclear protein 2
I: Gamma-aminobutyric acid receptor-associated protein
J: Tumor protein p53-inducible nuclear protein 2


Theoretical massNumber of molelcules
Total (without water)78,51010
Polymers78,51010
Non-polymers00
Water2,306128
1
A: Gamma-aminobutyric acid receptor-associated protein
B: Tumor protein p53-inducible nuclear protein 2


Theoretical massNumber of molelcules
Total (without water)15,7022
Polymers15,7022
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1230 Å2
ΔGint-9 kcal/mol
Surface area7560 Å2
MethodPISA
2
C: Gamma-aminobutyric acid receptor-associated protein
D: Tumor protein p53-inducible nuclear protein 2


Theoretical massNumber of molelcules
Total (without water)15,7022
Polymers15,7022
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1070 Å2
ΔGint-6 kcal/mol
Surface area7220 Å2
MethodPISA
3
E: Gamma-aminobutyric acid receptor-associated protein
F: Tumor protein p53-inducible nuclear protein 2


Theoretical massNumber of molelcules
Total (without water)15,7022
Polymers15,7022
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1270 Å2
ΔGint-9 kcal/mol
Surface area7210 Å2
MethodPISA
4
G: Gamma-aminobutyric acid receptor-associated protein
H: Tumor protein p53-inducible nuclear protein 2


Theoretical massNumber of molelcules
Total (without water)15,7022
Polymers15,7022
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area930 Å2
ΔGint-8 kcal/mol
Surface area6990 Å2
MethodPISA
5
I: Gamma-aminobutyric acid receptor-associated protein
J: Tumor protein p53-inducible nuclear protein 2


Theoretical massNumber of molelcules
Total (without water)15,7022
Polymers15,7022
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area920 Å2
ΔGint-8 kcal/mol
Surface area6970 Å2
MethodPISA
Unit cell
Length a, b, c (Å)51.898, 95.783, 138.390
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 2 through 11 or resid 13...
21(chain C and (resid 2 through 11 or resid 13...
31(chain E and (resid 2 through 11 or resid 13...
41(chain G and (resid 2 through 11 or resid 13...
51(chain I and (resid 2 through 11 or resid 13...
12(chain B and resid 29 through 41)
22chain F

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111LYSLYSPHEPHE(chain A and (resid 2 through 11 or resid 13...AA2 - 114 - 13
121LYSLYSALAALA(chain A and (resid 2 through 11 or resid 13...AA13 - 3915 - 41
131ILEILELEULEU(chain A and (resid 2 through 11 or resid 13...AA41 - 7043 - 72
141ALAALAGLYGLY(chain A and (resid 2 through 11 or resid 13...AA72 - 9274 - 94
161HISHISVALVAL(chain A and (resid 2 through 11 or resid 13...AA98 - 114100 - 116
211LYSLYSPHEPHE(chain C and (resid 2 through 11 or resid 13...CC2 - 114 - 13
221LYSLYSALAALA(chain C and (resid 2 through 11 or resid 13...CC13 - 3915 - 41
231ILEILELEULEU(chain C and (resid 2 through 11 or resid 13...CC41 - 7043 - 72
241ALAALAALAALA(chain C and (resid 2 through 11 or resid 13...CC7274
251HISHISVALVAL(chain C and (resid 2 through 11 or resid 13...CC98 - 114100 - 116
311LYSLYSPHEPHE(chain E and (resid 2 through 11 or resid 13...EE2 - 114 - 13
321LYSLYSALAALA(chain E and (resid 2 through 11 or resid 13...EE13 - 3915 - 41
331ILEILELEULEU(chain E and (resid 2 through 11 or resid 13...EE41 - 7043 - 72
341ALAALAGLYGLY(chain E and (resid 2 through 11 or resid 13...EE72 - 9274 - 94
351LEULEUTYRTYR(chain E and (resid 2 through 11 or resid 13...EE94 - 9596 - 97
361HISHISVALVAL(chain E and (resid 2 through 11 or resid 13...EE98 - 114100 - 116
411LYSLYSPHEPHE(chain G and (resid 2 through 11 or resid 13...GG2 - 114 - 13
421LYSLYSALAALA(chain G and (resid 2 through 11 or resid 13...GG13 - 3915 - 41
431ILEILELEULEU(chain G and (resid 2 through 11 or resid 13...GG41 - 7043 - 72
441ALAALAGLYGLY(chain G and (resid 2 through 11 or resid 13...GG72 - 9274 - 94
461HISHISVALVAL(chain G and (resid 2 through 11 or resid 13...GG98 - 114100 - 116
511LYSLYSPHEPHE(chain I and (resid 2 through 11 or resid 13...II2 - 114 - 13
521LYSLYSALAALA(chain I and (resid 2 through 11 or resid 13...II13 - 3915 - 41
531ILEILELEULEU(chain I and (resid 2 through 11 or resid 13...II41 - 7043 - 72
541ALAALAGLYGLY(chain I and (resid 2 through 11 or resid 13...II72 - 9274 - 94
551LEULEUTYRTYR(chain I and (resid 2 through 11 or resid 13...II94 - 9596 - 97
561HISHISVALVAL(chain I and (resid 2 through 11 or resid 13...II98 - 114100 - 116
112GLYGLYPROPRO(chain B and resid 29 through 41)BB29 - 411 - 13
212GLYGLYPROPROchain FFF29 - 411 - 13

NCS ensembles :
ID
1
2

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Components

#1: Protein
Gamma-aminobutyric acid receptor-associated protein / GABA(A) receptor-associated protein / MM46


Mass: 14086.176 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GABARAP, FLC3B, HT004 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: O95166
#2: Protein/peptide
Tumor protein p53-inducible nuclear protein 2 / Diabetes and obesity-regulated gene / p53-inducible protein U / PIG-U


Mass: 1615.738 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TP53INP2, C20orf110, DOR, PINH / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q8IXH6
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 128 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.23 Å3/Da / Density % sol: 44.93 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / Details: 25% PEG3350, 0.1M Na-citrate, 5% isopropanol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.9775 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 1, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9775 Å / Relative weight: 1
ReflectionResolution: 2.096→26.253 Å / Num. obs: 37232 / % possible obs: 90.03 % / Redundancy: 5.2 % / CC1/2: 0.997 / Net I/σ(I): 10.78
Reflection shellResolution: 2.096→2.171 Å / Num. unique obs: 1455 / CC1/2: 0.222

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Processing

Software
NameVersionClassification
PHENIX1.14_3260refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.096→26.253 Å / SU ML: 0.31 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 29.42 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2635 2000 5.37 %
Rwork0.197 35213 -
obs0.2005 37213 90.1 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 123.67 Å2 / Biso mean: 57.1633 Å2 / Biso min: 22.02 Å2
Refinement stepCycle: final / Resolution: 2.096→26.253 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5242 0 0 128 5370
Biso mean---51.53 -
Num. residues----624
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A2689X-RAY DIFFRACTION6.842TORSIONAL
12C2689X-RAY DIFFRACTION6.842TORSIONAL
13E2689X-RAY DIFFRACTION6.842TORSIONAL
14G2689X-RAY DIFFRACTION6.842TORSIONAL
15I2689X-RAY DIFFRACTION6.842TORSIONAL
21B116X-RAY DIFFRACTION6.842TORSIONAL
22F116X-RAY DIFFRACTION6.842TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.0963-2.14870.329460.326181230
2.1487-2.20680.37870.3099154156
2.2068-2.27170.35631280.2988223380
2.2717-2.34490.34391460.2872258794
2.3449-2.42870.33221530.2578269999
2.4287-2.52590.32361580.24762768100
2.5259-2.64070.31751570.24012759100
2.6407-2.77980.30831580.21832773100
2.7798-2.95370.30851570.2212782100
2.9537-3.18140.27611580.21012788100
3.1814-3.50090.25821600.19292802100
3.5009-4.0060.23921600.1762834100
4.006-5.04130.20611620.14852847100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.31460.4924-0.79281.93941.05391.4330.0701-0.11890.21850.5782-0.35990.4148-0.7417-0.10890.00020.39470.0585-0.01480.4173-0.02810.4033-9.43833.06729.1727
22.32390.07220.01721.90450.61582.9232-0.02060.37670.0034-0.1464-0.07750.2523-0.1709-0.1141-00.2496-0.030.0120.30320.00420.3545-6.7806-5.746916.0576
30.5266-0.7542.13231.1222-3.14688.8488-0.29950.68570.19210.3118-0.15350.4634-0.8528-0.6439-0.31260.1439-0.05440.05850.4497-0.14940.624-17.6453-8.860926.8885
40.1179-0.4199-0.49151.70492.30763.5934-0.1277-0.2546-1.1211-0.15270.12050.08091.52860.99720.03310.5170.05290.0610.3455-0.05470.6165-10.2494-20.076621.1942
50.5303-0.0701-0.29190.2631-0.25320.45270.0642-0.16250.62930.12940.4584-1.2201-0.11961.08890.0020.6320.01720.01930.5195-0.02790.427111.21924.840630.8823
60.5144-0.09670.44761.43650.2090.4224-0.1663-0.48190.31160.9909-0.44560.6115-0.1578-0.3583-0.03920.47220.0697-0.05050.3812-0.05770.54152.464315.996535.208
70.8789-0.21260.14021.3513-0.55420.83260.06950.42450.749-0.4696-0.1434-0.715-0.59230.6022-0.09610.4927-0.0951-0.09690.3355-0.0660.520410.351218.941526.5613
80.95260.9027-1.07031.1621-0.71381.9692-0.4054-0.47980.5258-0.02950.4093-0.3842-0.670.3348-0.0030.4588-0.0348-0.07150.5918-0.06730.532417.509114.540222.6207
91.9079-1.9340.71995.24532.6923.8061.32050.10610.319-1.81090.4935-0.17260.11331.00980.99920.7475-0.26170.03030.6347-0.00370.47319.000719.841816.9263
100.6999-0.755-0.08120.8999-0.26420.9116-0.29890.77180.1735-0.26570.2519-0.25810.02530.62640.00040.3572-0.11520.04930.71210.02890.503316.895713.214113.4454
110.3669-0.0063-0.45960.06480.02890.5771-0.0140.5751-0.1768-0.64280.08980.45060.2904-1.08350.00450.4121-0.07770.00990.37170.0090.44652.967617.494518.4461
120.74160.23430.89650.69180.01481.1044-0.0763-0.0544-0.1340.0898-0.0008-0.148-0.11610.37970.00140.3834-0.0009-0.03520.308-0.02460.36517.045611.578124.9097
130.0786-0.05340.05710.1240.04660.1605-0.2726-1.188-1.4018-0.52590.433-0.6088-0.17740.81170.00460.65560.0115-0.05560.68720.07060.775617.7073.116313.3032
143.3376-2.3092-2.36961.6131.66261.7073-0.99340.02720.80030.08750.7595-0.0018-0.01730.9134-0.02230.7635-0.1824-0.08840.4888-0.0340.687815.273223.268831.237
150.32230.09270.1950.67910.57060.47890.6064-1.3311-0.29720.6685-0.1107-0.40690.88640.92820.02220.35830.0315-0.16230.5244-0.02830.536620.8163-16.020546.4111
161.6879-1.66520.89541.8311-1.35031.6812-0.553-0.05580.4343-0.14180.768-1.04-1.19940.67150.05720.5327-0.2101-0.04010.5047-0.11810.61522.3015-3.17839.6018
172.77520.74671.70371.87681.53722.39330.14140.1048-0.3123-0.2404-0.2501-0.57130.2290.1719-0.0020.2523-0.0557-0.02160.34050.08070.485517.6283-19.511535.9922
180.0793-0.03520.09780.46630.20580.22820.10490.28590.3965-0.0598-0.0436-0.1952-0.27440.24400.2969-0.0070.04140.38250.02680.403514.4703-12.861628.4203
190.11450.0152-0.25630.4960.02470.4386-0.0005-0.1487-0.4714-0.63010.2309-0.02030.7834-0.0163-0.0010.44550.0049-0.05360.34890.06260.446312.6183-23.498428.2784
201.6629-1.9825-0.13522.20010.53593.6121-0.2861-0.2845-0.40340.3550.19330.48181.3103-0.1667-0.02890.4521-0.11440.00760.46520.0780.4556.9139-23.967634.3216
211.21910.42051.0171.0803-0.91672.1376-0.0072-0.3518-0.13380.3976-0.020.06950.1145-0.40130.00030.3666-0.0407-0.01720.40050.0130.366210.4105-14.747639.7027
223.23961.60033.32492.33090.17014.59110.35031.1124-0.8706-0.23460.4226-1.09570.76360.74060.21440.2879-0.08580.07320.4934-0.13940.540322.2663-13.524826.557
230.3309-0.0260.05770.1924-0.2080.2151-0.0047-0.2718-0.09530.0769-0.50020.46490.50260.16970.00270.445-0.01320.0160.5861-0.00110.366812.4725-6.591752.4747
240.742-0.2003-1.40832.7878-0.45172.9343-0.2038-0.03830.21780.43720.06470.141-1.3391-2.1566-0.74670.76810.327-0.11670.90330.10320.31590.00091.071549.5862
252.0954-0.76760.70040.6911-0.30240.24570.25090.6810.7448-0.0949-0.41740.1681-0.2231-1.5472-0.09880.27530.2937-0.0730.9139-0.01780.4231-0.5294-5.666859.2473
261.11730.63840.22561.0135-1.22293.16110.3478-0.7256-0.3921-0.0868-0.31740.11010.7083-0.8703-0.0010.5637-0.0314-0.06910.6857-0.04510.47773.6993-12.865965.3788
271.3297-0.26920.11540.57690.48521.5560.3214-0.1814-0.44550.2765-0.34090.1342-0.0085-0.8781-00.6704-0.0834-0.08410.53540.01830.46028.1519-10.0671.7387
284.39972.3011-1.6041.2989-0.92140.65370.1898-0.56690.1934-0.6863-1.06250.3119-2.4143-0.564-0.12111.01480.2357-0.03030.585-0.04180.571.73742.520265.788
290.7096-0.5295-0.90490.52960.45011.15770.2097-0.4666-0.29750.13040.03590.46920.0834-0.9570.00360.58810.0305-0.06640.4402-0.03930.44476.762-1.922659.1614
300.072-0.0110.01460.0917-0.060.0362-0.1867-0.3884-0.9072-0.25160.8818-1.58660.0391-0.10270.00510.7463-0.0079-0.16790.87670.07430.811317.8516-10.505770.904
310.188-0.2304-0.09520.2750.11480.04540.96680.5639-0.4546-0.2537-0.6384-0.789-0.8818-0.27510.01720.5785-0.0998-0.04090.9350.09820.4445-5.0455-10.981758.1547
320.2783-0.13870.2740.1373-0.06980.274-0.71010.0550.6611-0.39410.96650.1289-0.4413-0.10250.00130.840.051-0.17570.53030.06140.911810.993516.160258.8869
333.41141.4389-0.26150.94080.16180.2073-0.30381.72690.4663-1.52030.20090.34730.3092-0.193-0.01161.0711-0.0099-0.07540.68860.10620.373715.720711.952945.8527
343.11950.33181.2922.2856-0.58912.4507-0.26990.28830.2085-0.5953-0.1393-0.0142-0.97950.88380.00060.7807-0.0458-0.06180.6330.13380.610626.385216.301560.732
352.481-1.15720.90633.5435-2.59412.62680.08730.0533-0.1067-0.3812-0.1405-0.0240.15080.21250.00030.5004-0.0278-0.07130.43190.02020.477623.80499.520864.0672
360.00510.17730.05915.3421.70160.54210.05320.90530.1954-0.75430.02-1.3937-0.51290.49310.13750.7675-0.35490.10811.03850.13150.612528.821619.229951.9756
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 24 )A1 - 24
2X-RAY DIFFRACTION2chain 'A' and (resid 25 through 114 )A25 - 114
3X-RAY DIFFRACTION3chain 'B' and (resid 29 through 37 )B29 - 37
4X-RAY DIFFRACTION4chain 'B' and (resid 38 through 42 )B38 - 42
5X-RAY DIFFRACTION5chain 'C' and (resid 1 through 10 )C1 - 10
6X-RAY DIFFRACTION6chain 'C' and (resid 11 through 24 )C11 - 24
7X-RAY DIFFRACTION7chain 'C' and (resid 25 through 35 )C25 - 35
8X-RAY DIFFRACTION8chain 'C' and (resid 36 through 56 )C36 - 56
9X-RAY DIFFRACTION9chain 'C' and (resid 57 through 67 )C57 - 67
10X-RAY DIFFRACTION10chain 'C' and (resid 68 through 90 )C68 - 90
11X-RAY DIFFRACTION11chain 'C' and (resid 91 through 98 )C91 - 98
12X-RAY DIFFRACTION12chain 'C' and (resid 99 through 110 )C99 - 110
13X-RAY DIFFRACTION13chain 'C' and (resid 111 through 116 )C111 - 116
14X-RAY DIFFRACTION14chain 'D' and (resid 31 through 39 )D31 - 39
15X-RAY DIFFRACTION15chain 'E' and (resid 1 through 10 )E1 - 10
16X-RAY DIFFRACTION16chain 'E' and (resid 11 through 24 )E11 - 24
17X-RAY DIFFRACTION17chain 'E' and (resid 25 through 47 )E25 - 47
18X-RAY DIFFRACTION18chain 'E' and (resid 48 through 56 )E48 - 56
19X-RAY DIFFRACTION19chain 'E' and (resid 57 through 68 )E57 - 68
20X-RAY DIFFRACTION20chain 'E' and (resid 69 through 90 )E69 - 90
21X-RAY DIFFRACTION21chain 'E' and (resid 91 through 117 )E91 - 117
22X-RAY DIFFRACTION22chain 'F' and (resid 29 through 41 )F29 - 41
23X-RAY DIFFRACTION23chain 'G' and (resid 1 through 10 )G1 - 10
24X-RAY DIFFRACTION24chain 'G' and (resid 11 through 24 )G11 - 24
25X-RAY DIFFRACTION25chain 'G' and (resid 25 through 35 )G25 - 35
26X-RAY DIFFRACTION26chain 'G' and (resid 36 through 68 )G36 - 68
27X-RAY DIFFRACTION27chain 'G' and (resid 69 through 90 )G69 - 90
28X-RAY DIFFRACTION28chain 'G' and (resid 91 through 98 )G91 - 98
29X-RAY DIFFRACTION29chain 'G' and (resid 99 through 110 )G99 - 110
30X-RAY DIFFRACTION30chain 'G' and (resid 111 through 116 )G111 - 116
31X-RAY DIFFRACTION31chain 'H' and (resid 34 through 39 )H34 - 39
32X-RAY DIFFRACTION32chain 'I' and (resid 1 through 10 )I1 - 10
33X-RAY DIFFRACTION33chain 'I' and (resid 11 through 24 )I11 - 24
34X-RAY DIFFRACTION34chain 'I' and (resid 25 through 68 )I25 - 68
35X-RAY DIFFRACTION35chain 'I' and (resid 69 through 114 )I69 - 114
36X-RAY DIFFRACTION36chain 'J' and (resid 35 through 39 )J35 - 39

+
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