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- PDB-8pxk: Structure of NADH-DEPENDENT FERREDOXIN REDUCTASE, BPHA4, solved a... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8pxk | ||||||
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Title | Structure of NADH-DEPENDENT FERREDOXIN REDUCTASE, BPHA4, solved at wavelength 5.76 A | ||||||
![]() | Ferredoxin reductase | ||||||
![]() | OXIDOREDUCTASE / BPHA4 | ||||||
Function / homology | ![]() oxidoreductase activity, acting on NAD(P)H / flavin adenine dinucleotide binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | El Omari, K. / Duman, R. / Mykhaylyk, V. / Orr, C. / Senda, M. / Matsugaki, N. / Kawano, Y. / Wagner, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Experimental phasing opportunities for macromolecular crystallography at very long wavelengths. Authors: El Omari, K. / Duman, R. / Mykhaylyk, V. / Orr, C.M. / Latimer-Smith, M. / Winter, G. / Grama, V. / Qu, F. / Bountra, K. / Kwong, H.S. / Romano, M. / Reis, R.I. / Vogeley, L. / Vecchia, L. / ...Authors: El Omari, K. / Duman, R. / Mykhaylyk, V. / Orr, C.M. / Latimer-Smith, M. / Winter, G. / Grama, V. / Qu, F. / Bountra, K. / Kwong, H.S. / Romano, M. / Reis, R.I. / Vogeley, L. / Vecchia, L. / Owen, C.D. / Wittmann, S. / Renner, M. / Senda, M. / Matsugaki, N. / Kawano, Y. / Bowden, T.A. / Moraes, I. / Grimes, J.M. / Mancini, E.J. / Walsh, M.A. / Guzzo, C.R. / Owens, R.J. / Jones, E.Y. / Brown, D.G. / Stuart, D.I. / Beis, K. / Wagner, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 90.8 KB | Display | ![]() |
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PDB format | ![]() | 66.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 675.8 KB | Display | ![]() |
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Full document | ![]() | 676.6 KB | Display | |
Data in XML | ![]() | 15.2 KB | Display | |
Data in CIF | ![]() | 20.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8pwnC ![]() 8px0C ![]() 8px1C ![]() 8px4C ![]() 8px5C ![]() 8px7C ![]() 8px9C ![]() 8pxcC ![]() 8pxgC ![]() 8pxhC ![]() 8pxjC ![]() 8pxlC ![]() 8pyvC ![]() 8pyzC ![]() 8pz4C ![]() 8pz5C C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 43221.184 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-FAD / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.74 Å3/Da / Density % sol: 55.13 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: Sodium formate Acetate buffer, pH 4.6 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 12M / Detector: PIXEL / Date: Dec 8, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 5.767 Å / Relative weight: 1 |
Reflection | Resolution: 3.77→85.03 Å / Num. obs: 4614 / % possible obs: 86.62 % / Redundancy: 11.9 % / CC1/2: 0.995 / Rmerge(I) obs: 0.1448 / Rpim(I) all: 0.04015 / Rrim(I) all: 0.1507 / Net I/σ(I): 21.38 |
Reflection shell | Resolution: 3.77→3.905 Å / Num. unique obs: 373 / CC1/2: 0.987 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.146 Å2
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Refinement step | Cycle: 1 / Resolution: 3.77→85.03 Å
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Refine LS restraints |
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